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Titolo:
Inhibition of cortisol secretion in dispersed head kidney cells of rainbowtrout (Oncorhynchus mykiss) by endosulfan, an organochlorine pesticide
Autore:
Leblond, VS; Bisson, M; Hontela, A;
Indirizzi:
Univ Quebec, Dept Sci Biol, Montreal, PQ H3C 3P8, Canada Univ Quebec Montreal PQ Canada H3C 3P8 Biol, Montreal, PQ H3C 3P8, Canada Univ Quebec, TOXEN Res Ctr, Montreal, PQ H3C 3P8, Canada Univ Quebec Montreal PQ Canada H3C 3P8 Ctr, Montreal, PQ H3C 3P8, Canada
Titolo Testata:
GENERAL AND COMPARATIVE ENDOCRINOLOGY
fascicolo: 1, volume: 121, anno: 2001,
pagine: 48 - 56
SICI:
0016-6480(200101)121:1<48:IOCSID>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PITUITARY-INTERRENAL AXIS; PERCH PERCA-FLAVESCENS; IN-VIVO; FUNCTIONAL INTEGRITY; MILL EFFLUENT; ACTH; STRESS; RESPONSIVENESS; TISSUE; EXPOSURE;
Keywords:
cortisol; interrenal cells; endosulfan; ACTH; dbcAMP; LDH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Hontela, A Univ Quebec, Dept Sci Biol, CP 8888,Succ Ctr Ville, Montreal, PQ H3C 3P8, Canada Univ Quebec CP 8888,Succ Ctr Ville Montreal PQ Canada H3C3P8 a
Citazione:
V.S. Leblond et al., "Inhibition of cortisol secretion in dispersed head kidney cells of rainbowtrout (Oncorhynchus mykiss) by endosulfan, an organochlorine pesticide", GEN C ENDOC, 121(1), 2001, pp. 48-56

Abstract

The effects of endosulfan, an organochlorine pesticide, on cortisol secretion in vitro were investigated in enzymatically dispersed head kidney cellsof rainbow trout, Oncorhynchus mykiss. First, the conditions for maximal cortisol secretion were characterized by selecting the optimal concentrations of adrenocorticotropin (ACTH) (1 IU/ml) and N-6, 2'-o-dibutyryladenosine 3':5'-cyclic monophosphate (dbcAMP) (2 mM), incubation temperature (15 degrees) and time period (60 min for ACTH, 120 min for dbcAMP), number of cellsper incubation well (75 x 10(6)/ml), and osmolarity of the medium (270 mosmol/L). Exposure of head kidney cells to endosulfan decreased ACTH- or dbcAMP-stimulated cortisol secretion and cell viability in a concentration-dependent pattern and the doses required to disrupt cortisol secretion were significantly lower than doses lethal to the head kidney cells. The median effective concentration of endosulfan (EC50, the dose that inhibits cortisol secretion by 50%) was 17.3 muM while the median lethal concentration (LC50, the dose that kills 50% of the cells) was 308 muM. Our study identified endosulfan as an environmental endocrine disrupting chemical that interferes with the normal secretory function of teleost interrenal steroidogenic cells. Multiple sites may be affected within the steroidogenic cells since dbcAMP could not restore cortisol secretion in endosulfan exposed cells. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:49:44