Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Connexin 26 enhances the bystander effect in HSVtk/GCV gene therapy for human bladder cancer by adenovirus/PLL/DNA gene delivery
Autore:
Tanaka, M; Fraizer, GC; De la Cerda, J; Cristiano, RJ; Liebert, M; Grossman, HB;
Indirizzi:
Univ Texas, MD Anderson Canc Ctr, Dept Urol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 anc Ctr, Dept Urol, Houston, TX 77030 USA Univ Texas, MD Anderson Canc Ctr, Dept Genitourinary Med Oncol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 ourinary Med Oncol, Houston, TX 77030 USA
Titolo Testata:
GENE THERAPY
fascicolo: 2, volume: 8, anno: 2001,
pagine: 139 - 148
SICI:
0969-7128(200101)8:2<139:C2ETBE>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAP JUNCTION GENES; TUMOR-SUPPRESSOR GENE; CELLS IN-VIVO; INTERCELLULAR COMMUNICATION; BRAIN-TUMORS; NATURAL-HISTORY; GROWTH-CONTROL; GLIOMA-CELLS; HELA-CELLS; EXPRESSION;
Keywords:
bladder cancer; connexin 26; adenovirus/PLL/DNA complexes; HSVtk/GCV gene therapy; bystander effect;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Grossman, HB Univ Texas, MD Anderson Canc Ctr, Dept Urol, Box 110,1515 Holcombe Blvd, Houston, TX 77030 USA Univ Texas Box 110,1515 Holcombe Blvd Houston TX USA 77030 SA
Citazione:
M. Tanaka et al., "Connexin 26 enhances the bystander effect in HSVtk/GCV gene therapy for human bladder cancer by adenovirus/PLL/DNA gene delivery", GENE THER, 8(2), 2001, pp. 139-148

Abstract

Herpes simplex thymidine kinase/ganciclovir (HSVtk/GCV) gene therapy has been used for the treatment of a variety of cancers. Its efficacy is enhanced by the bystander effect that helps overcome the delivery problems commonly observed in current gene therapy. Connexins encode proteins that produce gap junctions, which enable intercellular communication and the bystander effect. We previously demonstrated that decreased Cx 26 expression and loss of gap junctional intercellular communication were associated with human bladder cancer. To investigate the efficacy of the bystander effect in HSVtk/GCV gene therapy, the Cx 26 gene was introduced into UM-UC-3 and UM-UC-14 bladder cancer cell lines by an adenovirus poly-L-lysine conjugate using a multigenic expression plasmid that expressed both the HSVtk and Cx 26 genes. We found significantly increased cytotoxicity in HSVtk/GCV gene therapy after introduction of the HSVtk and Cx 26 genes together compared with the cytotoxicity seen after introduction of the HSVtk gene and LacZ genes in vitro and in vivo. Cytotoxicity correlated with Cx 26 expression and the induction of functional gap junctions. This study indicates that combination genetherapy with co-expression of the HSVtk and Cx 26 genes potentiates HSVtk/GCV gene therapy through the bystander effect.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 03:06:55