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Titolo:
Decrease of L-selectin expression on human CD34(+) cells on freeze-thawingand rapid recovery with short-term incubation
Autore:
Hattori, Y; Kato, H; Nitta, M; Takamoto, S;
Indirizzi:
Aichi Med Univ, Dept Transfus Med, Nagakute, Aichi 4801195, Japan Aichi Med Univ Nagakute Aichi Japan 4801195 agakute, Aichi 4801195, Japan Aichi Med Univ, Dept Internal Med 2, Nagakute, Aichi 4801195, Japan Aichi Med Univ Nagakute Aichi Japan 4801195 agakute, Aichi 4801195, Japan
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 1, volume: 29, anno: 2001,
pagine: 114 - 122
SICI:
0301-472X(200101)29:1<114:DOLEOH>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BONE-MARROW; HEMATOPOIETIC PROGENITOR CELLS; BLOOD STEM-CELLS; ADHESION MOLECULES; PERIPHERAL-BLOOD; PLATELET RECOVERY; STROMAL CELLS; G-CSF; FIBRONECTIN; MODULATION;
Keywords:
hematopoietic progenitor cell; L-selectin; cryopreservation; homing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Kato, H Aichi Med Univ, Dept Transfus Med, 21 Karimata, Nagakute, Aichi 4801195, Japan Aichi Med Univ 21 Karimata Nagakute Aichi Japan 4801195 195, Japan
Citazione:
Y. Hattori et al., "Decrease of L-selectin expression on human CD34(+) cells on freeze-thawingand rapid recovery with short-term incubation", EXP HEMATOL, 29(1), 2001, pp. 114-122

Abstract

Objective. In the homing process of hematopoietic progenitor cells (HPCs) to bone marrow, several adhesion molecules play important roles, However, HPCs are subjected to dramatic: alteration of freeze-thawing that could affect these molecules. In this study we investigated the effect of cryopreservation on the expression of adhesion molecules on HPCs,Methods, The expression of different adhesion molecules on CD34(+) cells was examined by flow cytometry using an immunofluorescence technique. Changes in expression before and after cryopreservation, and that of L-selectin on addition of dimethylsulfoxide (DMSO) or with serum incubation, were investigated. The relationship between expression and function was also determined using a transmigration assay system,Results. L-selectin expression on CD34(+) cells derived from mobilized peripheral blood (MPB), bone marrow (BM), and umbilical cord blood (CB) was significantly decreased from 37% to 23%, 48% to 11%, and 67% to 19%, respectively, by the freeze-thawing process, while the expression of other adhesionmolecules was not appreciably changed. Within 30 minutes of incubation with DMSO, L-selectin expression on CD34(+) cells significantly decreased from65% to 22%. Furthermore, this was completely prevented by the matrix metalloproteinase (MMP) inhibitor, KB-R8301, indicating that the loss of L-selectin was due to shedding mediated by an MMP. To determine if L-selectin expression can be upregulated after cryopreservation, thaw ed samples were cultured overnight with serum. Values were observed to return to or rise higherthan those of the fresh samples, this being particularly rapid and pronounced when the CD34(+) cells,were cocultured with the human BM stromal cell line, HS-27A, Therefore, this adhesion molecule could possibly be restored in vivo after transplantation in a way similar to the in vitro case. Conclusion. Despite considerable damage to HPCs during cryopreservation, changes in these cells are reversible, in line with successful restoration of hematopoiesis after transplantation. (C) 2001 International Society for Experimental Hematology. published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 09:57:54