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Titolo:
The fate of forage plant DNA in farm animals: a collaborative case-study investigating cattle and chicken fed recombinant plant material
Autore:
Einspanier, R; Klotz, A; Kraft, J; Aulrich, K; Poser, R; Schwagele, F; Jahreis, G; Flachowsky, G;
Indirizzi:
Tech Univ Munich, Inst Physiol, FML, D-85350 Freising, Germany Tech Univ Munich Freising Germany D-85350 FML, D-85350 Freising, Germany Univ Jena, Inst Ernahrung & Umwelt, D-07743 Jena, Germany Univ Jena JenaGermany D-07743 Ernahrung & Umwelt, D-07743 Jena, Germany Bundesforsch Anstalt Landwirtschaft, Inst Tierernahrung, D-38116 Braunschweig, Germany Bundesforsch Anstalt Landwirtschaft Braunschweig Germany D-38116 Germany Bundesanstalt Fleischforsch, Inst Chem & Phys, D-95326 Kulmbach, Germany Bundesanstalt Fleischforsch Kulmbach Germany D-95326 6 Kulmbach, Germany
Titolo Testata:
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
fascicolo: 2, volume: 212, anno: 2001,
pagine: 129 - 134
SICI:
1438-2377(2001)212:2<129:TFOFPD>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
BT MAIZE; MICE; PRODUCTS;
Keywords:
recombinant plants; DNA transfer; polymerase chain reaction; farm animals; Bacillus thuringiensis toxin-maize;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Einspanier, R Tech Univ Munich, Inst Physiol, FML, Weihenstephaner Berg 3,D-85350 Freising, Germany Tech Univ Munich Weihenstephaner Berg 3 Freising Germany D-85350
Citazione:
R. Einspanier et al., "The fate of forage plant DNA in farm animals: a collaborative case-study investigating cattle and chicken fed recombinant plant material", EUR FOOD RE, 212(2), 2001, pp. 129-134

Abstract

The fate of ingested recombinant plant DNA in farm animals (cattle and chicken) being fed a diet containing conventional maize or recombinant Bacillus thuringiensis toxin-maize (Bt-maize) is described. The probability of thedetection by polymerase chain reaction of chloroplast-specific gene fragments of different lengths (199 bp and 532 bp) and a Pt-maize-specific fragment [truncated version of CryIA(b)] is shown. First data indicated that onlyshort DNA fragments (< 200 bp) derived from plant chloroplasts could be detected in the blood lymphocytes of cows. In all other cattle organs investigated (muscle, liver, spleen, kidney) plant DNAs were not found, except forfaint signals in milk. Furthermore, Pt-gene fragments possibly recording the uptake of recombinant maize, were not detected in any sample from cattle. However, in all chicken tissues (muscle, liver, spleen, kidney) the shortmaize chloroplast gene fragment was amplified. In contrast to this, no foreign plant DNA fragments were found in eggs. Pt-gene specific constructs originating from recombinant Pt-maize were not detectable in any of these poultry samples either.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:20:34