Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Steroidogenic factor 1 (SF1) is essential for pituitary gonadotrope function
Autore:
Zhao, LP; Bakke, M; Krimkevich, Y; Cushman, LJ; Parlow, AF; Camper, SA; Parker, KL;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Internal Med, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 r, Dept Internal Med, Dallas, TX 75390 USA Univ Texas, SW Med Ctr, Dept Pharmacol, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 Ctr, Dept Pharmacol, Dallas, TX 75390 USA Univ Michigan, Dept Human Genet, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 Human Genet, Ann Arbor, MI 48109 USA Harbor UCLA Med Ctr, Torrance, CA 90509 USA Harbor UCLA Med Ctr Torrance CA USA 90509 Med Ctr, Torrance, CA 90509 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 2, volume: 128, anno: 2001,
pagine: 147 - 154
SICI:
0950-1991(200101)128:2<147:SF1(IE>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOLLICLE-STIMULATING-HORMONE; VENTROMEDIAL HYPOTHALAMIC NUCLEUS; BETA-SUBUNIT PROMOTER; FACTOR-I; LUTEINIZING-HORMONE; TARGETED DISRUPTION; GENE-EXPRESSION; TRANSGENIC MICE; RECEPTOR GENE; BINDING-SITE;
Keywords:
hypogonadotropic hypogonadism; orphan nuclear receptor; gonadotropins; tissue-specific knockout; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Parker, KL Univ Texas, SW Med Ctr, Dept Internal Med, Dallas, TX 75390 USAUniv Texas Dallas TX USA 75390 ernal Med, Dallas, TX 75390 USA
Citazione:
L.P. Zhao et al., "Steroidogenic factor 1 (SF1) is essential for pituitary gonadotrope function", DEVELOPMENT, 128(2), 2001, pp. 147-154

Abstract

Knockout mice lacking the orphan nuclear receptor steroidogenic factor 1 (SF1) exhibit a complex endocrine phenotype that includes adrenal and gonadal agenesis, impaired expression of pituitary gonadotropins, and absence of the ventromedial hypothalamic nucleus (VMH). These multiple defects complicate efforts to delineate primary versus secondary effects of SF1 deficiencyin different tissues, such that its direct role in gonadotropes remains uncertain. To define this role, we have expressed Cre recombinase driven by the promoter region of the common alpha subunit of glycoprotein hormones (alpha GSU), thereby inactivating a loxP-modified SF1 locus in the anterior pituitary gland. Although pituitary-specific SF1 knockout mice were fully viable, they were sterile and failed to develop normal secondary sexual characteristics. Their adrenal glands and VMH appeared normal histologically, buttheir testes and ovaries were severely hypoplastic, alpha GSU-Cre, loxP mice had normal levels of most pituitary hormones, but had markedly decreasedexpression of LH and FSH. Treatment with exogenous gonadotropins stimulated gonadal steroidogenesis, inducing germ cell maturation in males and follicular and uterine maturation in females - establishing that the gonads can respond to gonadotropins, The pituitary-specific SF1 knockout mice are a novel genetic model of hypogonadotropic hypogonadism that establishes essential role(s) of SF1 in pituitary gonadotropes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 21:45:07