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Titolo:
The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration
Autore:
Adams, RH; Diella, F; Hennig, S; Helmbacher, F; Deutsch, U; Klein, R;
Indirizzi:
European Mol Biol Lab, D-69117 Heidelberg, Germany European Mol Biol Lab Heidelberg Germany D-69117 117 Heidelberg, Germany Max Planck Inst Physiol & Clin Res, WG Kerckhoff Inst, D-6350 Bad Nauheim,Germany Max Planck Inst Physiol & Clin Res Bad Nauheim Germany D-6350 im,Germany
Titolo Testata:
CELL
fascicolo: 1, volume: 104, anno: 2001,
pagine: 57 - 69
SICI:
0092-8674(20010112)104:1<57:TCDOTL>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR TYROSINE KINASES; EPH RECEPTORS; CARDIOVASCULAR DEVELOPMENT; SPROUTING ANGIOGENESIS; COMMISSURAL AXONS; HINDBRAIN; GENE; ANGIOPOIETIN-1; SEGMENTATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Klein, R European Mol Biol Lab, Meyerhofstr 1, D-69117 Heidelberg, GermanyEuropean Mol Biol Lab Meyerhofstr 1 Heidelberg Germany D-69117 y
Citazione:
R.H. Adams et al., "The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration", CELL, 104(1), 2001, pp. 57-69

Abstract

The transmembrane ligand ephrinB2 and its cognate Eph receptor tyrosine kinases are important regulators of vascular morphogenesis. EphrinB2 may havean active signaling role, resulting in bi-directional signal transduction downstream of both ephrinB2 and Eph receptors. To separate the ligand and receptorlike functions of ephrinB2 in mice, we replaced the endogenous gene by cDNAs encoding either carboxyterminally truncated (ephrinB2(DeltaC)) or,as a control, full-length ligand (ephrinB2(WT)). While homozygous ephrinB2(WT/WT) animals were viable and fertile, loss of the ephrinB2 cytoplasmic domain resulted in midgestation lethality similar to ephrinB2 null mutants (ephrinB2(KO)). The truncated ligand was sufficient to restore guidance of migrating cranial neural crest cells, but ephrinB2(DeltaC/DeltaC) embryos showed defects in vasculogenesis and angiogenesis very similar to those observed in ephrinB2(KO/KO) animals. Our results indicate distinct requirements of functions mediated by the ephrinB carboxyterminus for developmental processes in the vertebrate embryo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 03:46:49