Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Increased retinoic acid responsiveness in lung carcinoma cells that are nonresponsive despite the presence of endogenous retinoic acid receptor (RAR)beta by expression of exogenous retinoid receptors retinoid X receptor alpha, RAR alpha, and RAR gamma
Autore:
Wan, HS; Hong, WK; Lotan, R;
Indirizzi:
Univ Texas, MD Anderson Canc Ctr, Dept Thorac Head & Neck Med Oncol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 d & Neck Med Oncol, Houston, TX 77030 USA
Titolo Testata:
CANCER RESEARCH
fascicolo: 2, volume: 61, anno: 2001,
pagine: 556 - 564
SICI:
0008-5472(20010115)61:2<556:IRARIL>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRONCHIAL EPITHELIAL-CELLS; SQUAMOUS DIFFERENTIATION; SELECTIVE RETINOIDS; GROWTH-INHIBITION; CANCER CELLS; MATRIX METALLOPROTEINASE-1; TARGETED DISRUPTION; RXR HETERODIMERS; BINDING DOMAIN; BREAST-CANCER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Lotan, R Univ Texas, MD Anderson Canc Ctr, Dept Thorac Head & Neck Med Oncol, Box 80,1515 Holcombe Blvd, Houston, TX 77030 USA Univ Texas Box 80,1515Holcombe Blvd Houston TX USA 77030 030 USA
Citazione:
H.S. Wan et al., "Increased retinoic acid responsiveness in lung carcinoma cells that are nonresponsive despite the presence of endogenous retinoic acid receptor (RAR)beta by expression of exogenous retinoid receptors retinoid X receptor alpha, RAR alpha, and RAR gamma", CANCER RES, 61(2), 2001, pp. 556-564

Abstract

Nuclear retinoic acid receptors (RARs) and retinoid X receptors (RXRs) arethought to mediate most of the effects of retinoids on cell growth and differentiation. Despite expressing abundant levels of RAR beta mRNA, lung adenocarcinoma H1792 cells are resistant to the growth-inhibitory effects of all-halls-retinoic acid, suggesting that they have a defect in retinoid signaling. To determine whether transfection of exogenous receptors can restoreretinoid responsiveness, we transiently transfected into H1792 cells coexpression vectors containing cDNAs of cell surface antigen CD7 and either RARalpha, RAR beta, RAR gamma, or RXR alpha, The cells were then treated withretinoids and incubated with 5'-bromo-2'-deoxyuridine. Cells that express exogenous receptor were identified using antibodies against CD7, and cells that synthesized DNA were identified with anti-5'-bromo-2'-deoxyuridine antibodies using secondary antibodies with red and green fluorescence, respectively. RXR alpha and RAR alpha enhanced growth inhibition by all-trans-retinoic acid or 9-cis-retinoic acid, whereas RAR gamma was less effective, andRAR beta was ineffective. The effects of the transfected receptors were associated with antagonism of activator protein 1 (AP-1) activity. Studies with RXR alpha deletion and point mutants indicated that growth suppression is: (a) dependent on intact DNA-binding and ligand-binding regions but not on the NH2-terminal region, which contains a ligand-independent transactivation function; (b) dependent on RXR homodimer formation and transactivation of RXR response element; and (c) associated with AP-1 antagonism. These results demonstrate that transfected receptors can restore responsiveness to retinoids by antagonizing AP-1 in H1792 cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 18:38:15