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Titolo:
Oligoclonal T-cell receptor usage of melanocyte differentiation antigen-reactive T cells in stage IV melanoma patients
Autore:
Schrama, D; Andersen, MH; Terheyden, P; Schroder, L; Pedersen, LO; Straten, PT; Becker, JC;
Indirizzi:
Univ Wurzburg, Dept Dermatol, D-97080 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97080 rmatol, D-97080 Wurzburg, Germany Danish Canc Soc, Div Canc Biol, Dept Tumor Cell Biol, DK-2100 Copenhagen, Denmark Danish Canc Soc Copenhagen Denmark DK-2100 , DK-2100 Copenhagen, Denmark
Titolo Testata:
CANCER RESEARCH
fascicolo: 2, volume: 61, anno: 2001,
pagine: 493 - 496
SICI:
0008-5472(20010115)61:2<493:OTRUOM>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPHOCYTES; TUMOR; FREQUENCY; RESPONSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Becker, JC Univ Wurzburg, Dept Dermatol, Josef Schneider Str 2,bldg 13, D-97080 Wurzburg, Germany Univ Wurzburg Josef Schneider Str 2,bldg 13 Wurzburg Germany D-97080
Citazione:
D. Schrama et al., "Oligoclonal T-cell receptor usage of melanocyte differentiation antigen-reactive T cells in stage IV melanoma patients", CANCER RES, 61(2), 2001, pp. 493-496

Abstract

Ex vivo ELISPOT analysis of peripheral blood lymphocytes obtained from stage IV melanoma patients demonstrated reactivity against peptides derived from MART-1 and gp100. However, the number of reactive T cells was <1% that of total lymphocytes as detected by flow cytometry using tetrameric MHC/peptide complexes. Despite this low frequency, we were able to directly isolatethese populations ex vivo by means of magnetic beads coated with MHC/peptide complexes and to subject these cells to T-cell receptor clonotype! mapping. This analysis revealed that the MART-1/A*0201- and gp100/A*0201-reactive T-cell populations are composed of oligoclonal T cells that engage several T-cell receptor <beta> chain families. Longitudinal studies using this approach may result in a better correlation between T-cell reactivity and thecourse of neoplastic disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:39:42