Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Virol A, a toxic trans-polyacetylenic alcohol of Cicuta virosa, selectively inhibits the GABA-induced Cl- current in acutely dissociated rat hippocampal CA1 neurons
Autore:
Uwai, K; Ohashi, K; Takaya, Y; Oshima, Y; Furukawa, K; Yamagata, K; Omura, T; Okuyama, S;
Indirizzi:
Tohoku Univ, Grad Sch Pharmaceut Sci, Lab Nat Prod Chem, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 em, Sendai, Miyagi 9808578, Japan Hirosaki Univ, Sch Med, Dept Pharmacol, Hirosaki, Aomori 0368562, Japan Hirosaki Univ Hirosaki Aomori Japan 0368562 rosaki, Aomori 0368562, Japan Tokyo Metropolitan Inst Neurosci, Dept Mol Neurobiol, Fuchu, Tokyo 1838526, Japan Tokyo Metropolitan Inst Neurosci Fuchu Tokyo Japan 1838526 1838526, Japan Taisho Pharmaceut Co Ltd, Med Res Labs, Lab 1, Omiya, Saitama 3308530, Japan Taisho Pharmaceut Co Ltd Omiya Saitama Japan 3308530 itama 3308530, Japan
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 889, anno: 2001,
pagine: 174 - 180
SICI:
0006-8993(20010119)889:1-2<174:VAATTA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR; BICUCULLINE; PICROTOXIN; MECHANISMS; RESPONSES;
Keywords:
GABA(A) receptor; virol A; hippocampal neuron;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Oshima, Y Tohoku Univ, Grad Sch Pharmaceut Sci, Lab Nat Prod Chem, Sendai,Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 , Miyagi 9808578, Japan
Citazione:
K. Uwai et al., "Virol A, a toxic trans-polyacetylenic alcohol of Cicuta virosa, selectively inhibits the GABA-induced Cl- current in acutely dissociated rat hippocampal CA1 neurons", BRAIN RES, 889(1-2), 2001, pp. 174-180

Abstract

The effects of virol A (VA), a toxic component of Cicuta virosa (water hemlock), on the GABA-induced Cl- current (I-GABA) in acutely dissociated rat hippocampal CAI neurons were investigated using whole-cell patch-clamp techniques. VA reversibly reduced I-GABA and the muscimol (Mus)-induced current(I-Mus) in a concentration-dependent manner. The IC50 values for VA against I-GABA and I-Mus were 9.6X10(-7) and 9.8X10(-7) M, respectively. VA shifted the EC50 value of I-GABA from 6.5x10(-6) to 2.1X10(-5) M, whereas it hadno effect on the maximum response, thereby suggesting that VA inhibited I-GABA in a competitive manner. VA had no apparent effect on current-voltage relationships for I-GABA thus indicating the lack of voltage-dependency. Onthe other hand, application of VA (10(-6) M) did not additionally reduce the I-GABA suppressed by >10(-5) M picrotoxin. VA but not bicuculline accelerated the decay phase of I-GABA as, was seen with picrotoxin. Moreover, pre-application of 10(-5) M VA reduced I-GABA. VA did not inhibit that inducedby glycine (10(-4) M). These results indicate that VA inhibits I-GABA by acting both on the GABA agonist site and on the CI channel of the GABA, receptor-channel complex. VA is a structurally novel type of compound that selectively inhibits the GABA(A) receptor-Cl- channel complexes in mammalian central nervous system neurons. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:22:26