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Titolo:
A rapid and cost-effective method for analysis of three common genetic risk factors for thrombosis
Autore:
Cutler, JA; Mitchell, MJ; Greenslade, K; Smith, MP; Savidge, GF;
Indirizzi:
St Thomas Hosp, Reference Ctr Haemostat & Thrombot Disorders, London SE1 7EH, England St Thomas Hosp London England SE1 7EH Disorders, London SE1 7EH, England
Titolo Testata:
BLOOD COAGULATION & FIBRINOLYSIS
fascicolo: 1, volume: 12, anno: 2001,
pagine: 33 - 36
SICI:
0957-5235(200101)12:1<33:ARACMF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-C; METHYLENETETRAHYDROFOLATE REDUCTASE; VENOUS THROMBOSIS; PROTHROMBIN GENE; MUTATION; DEFICIENCY; PREDISPOSITION; RESISTANCE; DATABASE; DISEASE;
Keywords:
thrombosis; risk factors; polymerase chain reaction; Guthrie spots; single-stranded conformational polymorphism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Cutler, JA St Thomas Hosp, Reference Ctr Haemostat & Thrombot Disorders, Lambeth Palace Rd, London SE1 7EH, England St Thomas Hosp Lambeth Palace Rd London England SE1 7EH ngland
Citazione:
J.A. Cutler et al., "A rapid and cost-effective method for analysis of three common genetic risk factors for thrombosis", BL COAG FIB, 12(1), 2001, pp. 33-36

Abstract

A simple, rapid and cost-effective method for the analysis of three of themost widely screened genetic risk factors for thrombosis has been established. The protocol developed uses blood spots stored on filter paper (Guthrie spots) as well as DNA extracted from anticoagulated blood. The use of Guthrie spots taken at birth enables the retrospective study of patients who develop thrombotic complications without necessitating resampling. Followingisolation of DNA, conventional fluorescence-labelled polymerase chain reaction (PCR) is performed using a thermostable DNA polymerase. Denatured, single-stranded PCR products are analysed on a semi-automated capillary-based genetic analyser, the data being stored electronically. This sensitive protocol obviates the need for endonuclease digestion and the associated gel running and documentation, and leads to a reduction in the recurrent costs oflaboratory consumables. Blood Coagul Fibrinolysis 12:33-36 (C) 2001 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:52:48