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Titolo:
Enhanced taxol production and release in Taxus chinensis cell suspension cultures with selected organic solvents and sucrose feeding
Autore:
Wang, CG; Wu, JY; Mei, XG;
Indirizzi:
Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China Hong Kong Polytech Univ Kowloon Hong Kong Peoples R China eoples R China Huazhong Univ Sci & Technol, Inst Biotechnol, Wuhan 430074, Peoples R China Huazhong Univ Sci & Technol Wuhan Peoples R China 430074 Peoples R China
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 1, volume: 17, anno: 2001,
pagine: 89 - 94
SICI:
8756-7938(200101/02)17:1<89:ETPARI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BENZOPHENANTHRIDINE ALKALOIDS; ANTHRAQUINONE PRODUCTION; ALGINATE IMMOBILIZATION; PACLITAXEL PRODUCTION; SHIKONIN PRODUCTION; METHYL JASMONATE; STIMULATION; ELICITATION; CALIFORNICA; EXTRACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Wu, JY Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China Hong Kong Polytech Univ Kowloon Hong Kong PeoplesR China R China
Citazione:
C.G. Wang et al., "Enhanced taxol production and release in Taxus chinensis cell suspension cultures with selected organic solvents and sucrose feeding", BIOTECH PR, 17(1), 2001, pp. 89-94

Abstract

Suspension culture of Taxus chinensis cells was carried out in aqueous-organic two-phase systems for the production and in situ solvent extraction oftaxol (paclitaxel). Three organic solvents, hexadecane, decanol, and dibutylphthalate, were tested at 5-20% (v/v) in the culture liquid. All of thesesolvents stimulated taxol release and the yield per cell, though decanol and higher concentrations of the other two solvents depressed biomass growthsignificantly. Ten percent dibutylphthalate was the optimal solvent for improving taxol production and release with minimal cell growth inhibition. The time of solvent addition to the culture also affected taxol production, with the addition during the late-log growth phase being most favorable. Byfeeding sucrose to the culture near the stationary growth phase, the cell growth and taxol production period was extended from 27 to 42 days. The combining of the two-phase culture and sucrose feeding increased the taxol yield by about 6-fold compared with the single-phase batch culture, to 36.0 +/- 3.5 mg/L, with up to 63% taxol released. This study shows that in situ solvent extraction combined with nutrient feeding is an effective process strategy for production and recovery of secondary metabolites in plant cell suspension culture.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 04:58:47