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Titolo:
Precursor balancing for metabolic engineering of lycopene production in Escherichia coli
Autore:
Farmer, WR; Liao, JC;
Indirizzi:
Univ Calif Los Angeles, Dept Chem Engn, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 1, volume: 17, anno: 2001,
pagine: 57 - 61
SICI:
8756-7938(200101/02)17:1<57:PBFMEO>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAROTENOID PIGMENT BIOSYNTHESIS; YEAST CANDIDA-UTILIS; PYRUVATE-KINASE; FOOD YEAST; POLYKETIDE SYNTHASE; ISOPRENOID PATHWAY; BETA-CAROTENE; EXPRESSION; GLUCOSE; PLANTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Liao, JC Univ Calif Los Angeles, Dept Chem Engn, Los Angeles, CA 90095 USAUniv Calif Los Angeles Los Angeles CA USA 90095 es, CA 90095 USA
Citazione:
W.R. Farmer e J.C. Liao, "Precursor balancing for metabolic engineering of lycopene production in Escherichia coli", BIOTECH PR, 17(1), 2001, pp. 57-61

Abstract

One issue that must be addressed in the rational design of metabolic pathways is the elimination of potential bottlenecks in the upstream pathways. We have reconstructed the isoprenoid pathway to overproduce the carotenoid lycopene in Escherichia coli. Here we show that the distribution between pyruvate and glyceraldehyde 3-phosphate (G3P), the originating precursors of the isoprenoid pathway, is a major factor that can limit isoprenoid production yields in E. coli. In particular, alterations in the central metabolism that redirect flux from pyruvate back to G3P enhance lycopene production, while alterations that channel carbon flux away from the G3P pool have the opposite effect. These results suggest that G3P may be limiting in the biosynthesis of lycopene, and modifications that achieve a more equitable distribution between the two precursors are able to increase the lycopene yield in metabolically engineered E. coli.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 13:29:01