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Titolo:
Development of assay system for immunoglobulin production regulatory factors using whole cell cultures of mouse splenocytes
Autore:
Takasugi, M; Tamura, Y; Tachibana, H; Sugano, M; Yamada, K;
Indirizzi:
Yamaguchi Prefectural Univ, Fac Human Life Sci, Yamaguchi 7538502, Japan Yamaguchi Prefectural Univ Yamaguchi Japan 7538502 aguchi 7538502, Japan Kyushu Univ, Grad Sch, Div Bioresource & Bioenvironm Sci, Higashi Ku, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 ci, Higashi Ku, Fukuoka 8128581, Japan Prefectural univ Kumamoto, Fac Environm & Symbiot Sci, Kumamoto 8628502, Japan Prefectural univ Kumamoto Kumamoto Japan 8628502 Kumamoto 8628502, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 1, volume: 65, anno: 2001,
pagine: 143 - 149
SICI:
0916-8451(200101)65:1<143:DOASFI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPRAGUE-DAWLEY RATS; SPLEEN LYMPHOCYTES; POLYCLONAL ACTIVATION; BETA-LACTOGLOBULIN; IGE PRODUCTION; BILE-ACID; LIPOPOLYSACCHARIDE; PHYTOHEMAGGLUTININ; INHIBITION; DISEASE;
Keywords:
adhesive cells; milk proteins; mitogens; immnoglobulin; splenocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Takasugi, M Yamaguchi Prefectural Univ, Fac Human Life Sci, 3-2-1 Sakurabatake, Yamaguchi 7538502, Japan Yamaguchi Prefectural Univ 3-2-1 Sakurabatake Yamaguchi Japan 7538502
Citazione:
M. Takasugi et al., "Development of assay system for immunoglobulin production regulatory factors using whole cell cultures of mouse splenocytes", BIOS BIOT B, 65(1), 2001, pp. 143-149

Abstract

We tried to establish an assay system for screening and assessment of immunoregulatory factors using whole cell cultures of mouse splenocytes and found that splenic adhesive cells markedly increased immunogobulin (Ig) production of splenocytes. In the absence of adhesive cells, lipopolysaccharides,pokeweed mitogen, and phytohemagglutinin stimulated the production of IgA,IgG, and IgM in a class-dependent manner. Adhesive cells increased more markedly Ig production of splenocytes stimulated with these mitogens, When mouse splenocytes were cultured with milk proteins in the absence of adhesivecells, lactoferrin, beta -lactoglobulin, alpha -casein, and beta -casein stimulated IgA and Ige production. Adhesive cells increased IgA production of splenocytes stimulated with milk proteins, especially, These results suggest that the assay system is useful for assessment of Ig production-regulating factors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:12:43