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Titolo:
A novel approach to studying the migratory morphology of embryonic mesenchymal cells
Autore:
Harkin, DG; Bianco, JI; Collins, N;
Indirizzi:
Griffith Univ, Sch Biomol & Biomed Sci, Nathan, Qld 4111, Australia Griffith Univ Nathan Qld Australia 4111 Sci, Nathan, Qld 4111, Australia
Titolo Testata:
BIOLOGY OF THE CELL
fascicolo: 7, volume: 92, anno: 2000,
pagine: 537 - 543
SICI:
0248-4900(200010)92:7<537:ANATST>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORNEAL FIBROBLASTS; CHEMOTACTIC FACTORS; DIVALENT-CATIONS; GROWTH-FACTOR; LOCOMOTION; POLARIZATION; MOTILITY; CULTURE; PDGF; CYTOSKELETON;
Keywords:
amoeboid movement; polarization; mesenchymal cells; PDGF; cornea;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Harkin, DG Griffith Univ, Sch Biomol & Biomed Sci, Nathan, Qld 4111, Australia Griffith Univ Nathan Qld Australia 4111 n, Qld 4111, Australia
Citazione:
D.G. Harkin et al., "A novel approach to studying the migratory morphology of embryonic mesenchymal cells", BIO CELL, 92(7), 2000, pp. 537-543

Abstract

A polarized morphology, defined by extension of an anterior pseudopod, is essential for the amoeboid migration of embryonic mesenchymal cells. Leukocytes adopt a similar morphology immediately following suspension in simple buffers containing chemotactic factors. Polarization in suspension therefore provides a rapid and sensitive screening assay for putative regulators ofleukocyte migration. The aim of the present study was to investigate whether this assay might also be used to study the motile behaviour of embryonicmesenchymal cells. Primary cultures of mesenchymal cells were established from explants of stage 28 chick embryo corneal-limbal stroma. Serum-starved, subconfluent cultures were harvested using ethylenediamine tetra-acetic acid and resuspended in Hanks' solution for up to 15 min at 37 degreesC. A variety of cell shapes, including spherical cells, blebbed cells, and cells with either non-polarized or polarized pseudopodia were observed. The proportions of cells with pseudopodia increased significantly over time. Treatment of cells with the chemotactic mitogen platelet derived growth factor-BE (PDGF-BB, homodimer isoform) suppressed blebbing and increased both pseudopod formation and polarization. Optimal polarization occurred in concentrations of PDGF-BB that are similar to those required for optimal chemotaxis (10 ng.mL(-1)). The polarization observed in the absence of PDGF-BB suggests that the migration of cells examined in this study might be controlled at least in part by some intrinsic mechanism. In addition, the strong polarization response to PDGF-BB confirms the role for this growth factor during corneal development. Observations of mesenchymal cell morphology in suspension, therefore provide novel data regarding the motile behaviour of embryonic cells. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 03:11:59