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Titolo:
CALCIUM AND PROTEIN-KINASE-C PLAY A SIGNIFICANT ROLE IN RESPONSE TO SHIGELLA TOXIN IN RABBIT ILEUM BOTH IN-VIVO AND IN-VITRO
Autore:
KAUR T; SINGH S; VERMA M; GANGULY NK;
Indirizzi:
POSTGRAD INST MED EDUC & RES,DEPT EXPT MED & BIOTECHNOL CHANDIGARH 160012 INDIA POSTGRAD INST MED EDUC & RES,DEPT EXPT MED & BIOTECHNOL CHANDIGARH 160012 INDIA POSTGRAD INST MED EDUC & RES,DEPT PEDIAT CHANDIGARH 160012 INDIA
Titolo Testata:
Biochimica et biophysica acta. Molecular basis of disease
fascicolo: 1, volume: 1361, anno: 1997,
pagine: 75 - 91
SICI:
0925-4439(1997)1361:1<75:CAPPAS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTEROTOXIN-INDUCED DIARRHEA; LAMBLIA INDUCED DIARRHEA; HEAT-STABLE ENTEROTOXIN; INDUCED FLUID SECRETION; ELECTROLYTE TRANSPORT; GIARDIA-LAMBLIA; SHIGA TOXIN; INTESTINAL SECRETION; ESCHERICHIA-COLI; RAT ENTEROCYTES;
Keywords:
SHIGELLA; CALCIUM ION; PROTEIN KINASE C; PROSTAGLANDIN; DIARRHEA; TOXIN; (RABBIT ILEUM);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
T. Kaur et al., "CALCIUM AND PROTEIN-KINASE-C PLAY A SIGNIFICANT ROLE IN RESPONSE TO SHIGELLA TOXIN IN RABBIT ILEUM BOTH IN-VIVO AND IN-VITRO", Biochimica et biophysica acta. Molecular basis of disease, 1361(1), 1997, pp. 75-91

Abstract

The role of second messengers in Shigella toxin (STx) induced fluid secretion in rabbit ileum was evaluated. In vivo and in vitro studies were carried out in presence or absence of following modulators: Ca2+ ionophore A23187 (15 mu M), l-verapamil (200 mu M), phorbol-12-myristate-13-acetate (PMA, 200 ng), 1-(5-isoquinolinyl-sulphonyl)-2-methyl-piperazine (H-7, 15 mu g) and indomethacin (20 mu M). In in vivo studies,the fluid accumulation into rabbit ileal loops in response to STx wasmeasured in presence or absence of these modulators. In in vitro studies, unidirectional fluxes of Na+ and Cl- were carried out in presenceor absence of these modulators. The addition of Ca2+ ionophore A23187along with STx further increases the amount of fluid already induced by STx. Whereas the presence of I-verapamil along with STx did not decrease the amount of fluid induced by STx. In vitro findings were in consonance with the in vivo studies. A significant increase in inositol triphosphate (IP3) levels was observed in enterocytes isolated from STx treated rabbit ileum. The addition of PMA into rabbit ileal loops inpresence of STx mimicked the effect of STx while the presence of H-7 reversed the secretion caused by STx to absorption. Similar results were obtained while determining unidirectional fluxes of Na+ and Cl- in presence of PMA and also with H-7. A significant increase in PKC levels was observed in the membrane fraction of enterocytes isolated from STx treated rabbit ileum as compared to control. Further a marked decrease in PKC levels was observed in the presence of H-7 in membrane fraction of enterocytes isolated from STx treated rabbit ileum. The addition of indomethacin into rabbit ileal loops reversed the secretion (caused by STx) to absorption. In vitro findings were in consonance with in vivo studies. Besides, there was a significant increase in PG-E levels in enterocytes isolated from STx treated rabbit ileum as compared to control. These findings suggested that STx induced enteritis involves the role of PKC, intracellular calcium stores and prostaglandins. The extracellular calcium pool probably does not play a significant rolein this process. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 04:31:31