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Titolo:
Conditionally immortalized cell lines as a new in vitro model for the study of barrier functions
Autore:
Terasaki, T; Hosoya, K;
Indirizzi:
Tohoku Univ, Grad Sch Pharmaceut Sci, Dept Mol Biopharm & Genet, Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ, New Ind Creat Hatchery Ctr, Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 Ku, Sendai, Miyagi 9808578, Japan Toyama Med & Pharmaceut Univ, Fac Pharmaceut Sci, Dept Pharmaceut Sci, Toyama 9300194, Japan Toyama Med & Pharmaceut Univ Toyama Japan 9300194 Toyama 9300194, Japan Japan Sci & Technol Corp, CREST, Osaka, Japan Japan Sci & Technol Corp Osaka Japan Technol Corp, CREST, Osaka, Japan
Titolo Testata:
BIOLOGICAL & PHARMACEUTICAL BULLETIN
fascicolo: 2, volume: 24, anno: 2001,
pagine: 111 - 118
SICI:
0918-6158(200102)24:2<111:CICLAA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-BRAIN-BARRIER; AMINO-ACID-TRANSPORT; EFFLUX INDEX METHOD; T-ANTIGEN GENE; RESISTANCE-ASSOCIATED PROTEIN; MULTISPECIFIC ORGANIC ANION; CAPILLARY ENDOTHELIAL-CELLS; IN-VIVO EVIDENCE; P-GLYCOPROTEIN; CEREBROSPINAL-FLUID;
Keywords:
blood-brain barrier; blood-retinal barrier; blood-cerebrospinal fluid barrier; transport functions; cell line; temperature-sensitive SV 40 large T-antigen;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
76
Recensione:
Indirizzi per estratti:
Indirizzo: Terasaki, T Tohoku Univ, Grad Sch Pharmaceut Sci, Dept Mol Biopharm & Genet, Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 Miyagi 9808578, Japan
Citazione:
T. Terasaki e K. Hosoya, "Conditionally immortalized cell lines as a new in vitro model for the study of barrier functions", BIOL PHAR B, 24(2), 2001, pp. 111-118

Abstract

Conditionally immortalized brain and retinal capillary endothelial and choroid plexus epithelial cell lines were established from a transgenic rat (Tg rat) and mouse (Tg mouse) harboring the temperature-sensitive simian virus 40 (ts SV 40) large T-antigen. These cell lines exhibit temperature-sensitive cell growth due to the expression of ts SV 40 large T-antigen. Mouse brain (TM-BBB) and rat brain (TR-BBB) and rat retinal (TR-iBRB) capillary endothelial cell lines appear to have a spindle-fiber shaped morphology and exhibit the typical endothelial markers, such as von Willebrand factor and acetylated low-density lipoprotein uptake. These cell lines express in vivo influx and efflux transporters, such as P-glycoprotein (P-gp) and GLUT1, which is capable of 3-O-methyl-D-glucose transport. TM-BBB cells are able to undergo efflux transport of cyclosporin A, which is a substrate for P-gp transport activity. They may also express oatp2 and exhibit dehydroepiandrosterone sulfate and digoxin uptake activity. TR-BBB cells express the mRNA ofmultidrug resistance associated protein 1 (MRP1) and a large neutral aminoacid transporter, which consists of LAT1 and 4F2hc. TR-iBRB cells exhibit pH-dependent L-lactic acid transport activity and express the mRNA of monocarboxylate transporter (MCT) 1 and 2. The choroid plexus epithelial cell line (TR-CSFB) has polygonal cell morphology, expresses the typical choroid plexus epithelial cell marker, transthyretin, and has Na+,K+-ATPase located on the apical side. TR-CSFB cells also exhibit amino acid transport activity which has been observed in vivo. These barrier cell lines established from the Tg rat and Tg mouse have in vitro transport functions and are good invitro models for drug transport to the brain and retina and as a screen for drugs which might be capable of delivery to the brain and retina.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 19:13:45