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Titolo:
Epidermal growth factor induces Gadd45 (growth arrest and DNA damage inducible protein) expression in A431 cells
Autore:
Fong, WF; Leung, CH; Lam, W; Wong, NS; Cheng, SH;
Indirizzi:
City Univ Hong Kong, Dept Biol & Chem, Bioact Prod Res Grp, Kowloon, Hong Kong, Peoples R China City Univ Hong Kong Kowloon Hong Kong Peoples R China g, Peoples R China Univ Hong Kong, Fac Med, Dept Biochem, Pokfulam, Hong Kong, Peoples R China Univ Hong Kong Pokfulam Hong Kong Peoples R China Kong, Peoples R China
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
fascicolo: 2, volume: 1517, anno: 2001,
pagine: 250 - 256
SICI:
0167-4781(20010126)1517:2<250:EGFIG(>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSTTRANSCRIPTIONAL REGULATION; INDUCED APOPTOSIS; CYCLE CHECKPOINT; CARCINOMA CELLS; KINASE-ACTIVITY; MESSENGER-RNA; INDUCTION; ACTIVATION; INHIBITION; RECEPTORS;
Keywords:
A431 cell; epidermal growth factor; apoptosis; Gadd45;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Fong, WF City Univ Hong Kong, Dept Biol & Chem, Bioact Prod Res Grp, Kowloon, Hong Kong, Peoples R China City Univ Hong Kong Kowloon Hong Kong Peoples R China s R China
Citazione:
W.F. Fong et al., "Epidermal growth factor induces Gadd45 (growth arrest and DNA damage inducible protein) expression in A431 cells", BBA-GENE ST, 1517(2), 2001, pp. 250-256

Abstract

Epidermal growth factor (EGF) receptor over-expressing, p53-deficient A431cells response to toxic dose of EGF by G1 arrest and apoptosis. Applying cDNA expression array technology we demonstrated that EGF increased the levels of Gadd45 mRNA. Northern blot and Western blot analyses confirmed that both Gadd45 mRNA and protein were increased. Concurrently half-lives of Gadd45 mRNA and protein also increased. Nuclear run-on experiments did not showa large increase of Gadd45 mRNA transcription rate. Gadd45 mRNA and protein started to increase after 1 h of EGF treatment and remained high for up to 10 h. We have also confirmed previous studies which showed that in EGF-stimulated A431 cells p21(Cip1/Waf1) (cyclin-dependent kinase interacting protein 1) was up-regulated within the same time frame. Thus it appears that in addition to inducing G2 arrest by directly disrupting Cdc2/Cyclin B1 complex in genotoxic-stressed cells, Gadd45 may also participate in G1 arrest in growth factor overexposed cells. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 19:14:43