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Titolo:
Improved analysis of dissolved carbohydrates in stream water with HPLC-PAD
Autore:
Cheng, XH; Kaplan, LA;
Indirizzi:
Stroud Water Res Ctr, Avondale, PA 19311 USA Stroud Water Res Ctr Avondale PA USA 19311 es Ctr, Avondale, PA 19311 USA
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 3, volume: 73, anno: 2001,
pagine: 458 - 461
SICI:
0003-2700(20010201)73:3<458:IAODCI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PULSED AMPEROMETRIC DETECTION; ORGANIC-MATTER; NATURAL-WATERS; NORTH-SEA; SUGARS; MONOSACCHARIDES; CHROMATOGRAPHY; SEAWATER; ACIDS; BLOOM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Cheng, XH Stroud Water Res Ctr, 970 Spencer Rd, Avondale, PA 19311 USA Stroud Water Res Ctr 970 Spencer Rd Avondale PA USA 19311 11 USA
Citazione:
X.H. Cheng e L.A. Kaplan, "Improved analysis of dissolved carbohydrates in stream water with HPLC-PAD", ANALYT CHEM, 73(3), 2001, pp. 458-461

Abstract

This paper describes improvements made to the determination of dissolved carbohydrates in stream water with high-performance liquid chromatography and pulsed amperometric detection (HPLC-PAD). We eliminated interference fromdissolved oxygen, separated xylose and mannose along with other molecules,reduced the chromatographic peak shift associated with carbonate accumulation in the column to less than 1% for all samples, and achieved an 8% increase in recovery of hydrolyzed carbohydrates by replacing ion-exchange desalting cartridges with evaporative removal of HCl under N-2, These modifications lowered detection limits to less than or equal to 0.4 nM for 10 different monosaccharides and enhanced our ability to follow the dynamics of thesemolecules in stream ecosystems. In the determination of dissolved free monosaccharides, average relative precision was 1.3% and recovery ranged from 92 to 109%, For dissolved total saccharides, average relative precision was3.3%. Concentrations were stable when filtered (0.2 mum) stream water was stored for 1 day at 22 degreesC, 1 week at 4 degreesC, or 1.5 months at -20degreesC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 11:46:33