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Titolo:
Adenovirus infection increases iNOS and peroxynitrite production in the lung
Autore:
Zsengeller, ZK; Ross, GF; Trapnell, BC; Szabo, C; Whitsett, JA;
Indirizzi:
Childrens Hosp, Med Ctr, Div Neonatol, Cincinnati, OH 45229 USA Childrens Hosp Cincinnati OH USA 45229 Neonatol, Cincinnati, OH 45229 USA Childrens Hosp, Med Ctr, Div Pulm Biol, Cincinnati, OH 45229 USA ChildrensHosp Cincinnati OH USA 45229 ulm Biol, Cincinnati, OH 45229 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 3, volume: 280, anno: 2001,
pagine: L503 - L511
SICI:
1040-0605(200103)280:3<L503:AIIIAP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; MEDIATED GENE-TRANSFER; CONDUCTANCE REGULATOR CDNA; CYSTIC-FIBROSIS; NONHUMAN-PRIMATES; SURFACTANT HOMEOSTASIS; AIRWAY EPITHELIUM; CFTR GENE; INFLAMMATION; THERAPY;
Keywords:
lung inflammation; surfactant; inducible nitric oxide synthase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Whitsett, JA Childrens Hosp, Med Ctr, Div Neonatol, 3333 Burnet Ave, Cincinnati, OH 45229 USA Childrens Hosp 3333 Burnet Ave Cincinnati OH USA 45229 29 USA
Citazione:
Z.K. Zsengeller et al., "Adenovirus infection increases iNOS and peroxynitrite production in the lung", AM J P-LUNG, 280(3), 2001, pp. L503-L511

Abstract

Host inflammatory and immune responses limit viral gene expression after administration of replication-deficient adenoviruses to the lung. The current study asks whether inducible nitric oxide synthase (iNOS) expression and peroxynitrite generation accompanied the inflammatory response following intratracheal administration of adenovirus. Pulmonary iNOS mRNA and protein were increased 2, 7, and 14 days following administration of 2 x 10(9) plaque-forming units of recombinant adenovirus (Av1Luc1) to BALB/c mice. Adenovirus infection was associated with a marked increase in nitrotyrosine staining. Intense nitrotyrosine staining was observed in alveolar macrophages, respiratory epithelial cells, conducting airways, and alveolar spaces 2 days postinfection. Two weeks after exposure to adenovirus, nitrotyrosine staining was detected within alveolar macrophages, suggesting adenovirus enhancedthe nitration of proteins that were subsequently taken up by alveolar macrophages. Western blot analysis using anti-nitrotyrosine antibody did not demonstrate accumulation of nitrated surfactant protein A (SP-A), although a small fraction of aggregated SP-A comigrated with a nitrotyrosine-positive protein. iNOS expression, peroxynitrite, and nitrotyrosine generation accompany and may contribute to inflammatory responses to adenovirus in the lung.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:38:56