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Titolo:
HIV type 1 molecular clones able to use the Bonzo/STRL-33 coreceptor for virus entry
Autore:
Zhang, YJ; Zhang, LQ; Ketas, T; Korber, BTM; Moore, JP;
Indirizzi:
Cornell Univ, Joan & Sanford I Weill Med Coll, Dept Microbiol & Immunol, New York, NY 10021 USA Cornell Univ New York NY USA 10021 biol & Immunol, New York, NY 10021 USA Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10021 USA Rockefeller Univ New York NY USA 10021 DS Res Ctr, New York, NY 10021 USA Los Alamos Natl Labs, Los Alamos, NM USA Los Alamos Natl Labs Los Alamos NM USA mos Natl Labs, Los Alamos, NM USA
Titolo Testata:
AIDS RESEARCH AND HUMAN RETROVIRUSES
fascicolo: 3, volume: 17, anno: 2001,
pagine: 217 - 227
SICI:
0889-2229(200102)17:3<217:HT1MCA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIMIAN-IMMUNODEFICIENCY-VIRUS; GP120 ENVELOPE GLYCOPROTEIN; CHEMOKINE RECEPTORS; CO-RECEPTORS; MULTIPLE CORECEPTORS; VIRAL PHENOTYPE; DIVERSE HUMAN; TROPIC HIV-1; CELLS; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Moore, JP Cornell Univ, Joan & Sanford I Weill Med Coll, Dept Microbiol & Immunol, W-805,1300 York Ave, New York, NY 10021 USA Cornell Univ W-805,1300 York Ave New York NY USA 10021 10021 USA
Citazione:
Y.J. Zhang et al., "HIV type 1 molecular clones able to use the Bonzo/STRL-33 coreceptor for virus entry", AIDS RES H, 17(3), 2001, pp. 217-227

Abstract

We describe the cloning of env genes from the mother-infant HIV-1 isolate pair P6-v3 and M6-v3. These viruses are unusual in that they can use the coreceptor Bonzo/STRL33 as well as CCR5 and, in the case of M6, CXCR4, to enter transfected cell lines in vitro. The phenotype of the parental isolates is generally reflected by the properties of the cloned env genes, when these are used in an Env-complementation assay of virus entry. Chimeric viruseswere also made that contain the env genes of P6-v3 and M6-v3 inserted intothe background of the infectious molecular clone, HIV-1 NL4-3. Some of thechimeric viruses derived from HIV-1 P6-v3 were able to use Bonzo for entryinto transfected cell lines, albeit to a lesser extent than they could useCCR5. There are some indications that one of these chimeric viruses, P6-v3-22-1, can use a coreceptor other than CCR5, perhaps Bonzo, to enter mitogen-stimulated PBMC, although only weakly. However, formal proof that this virus can use Bonzo in primary cells has not been obtained. The P6-v3-22-1 chimeric virus was unable to infect CD4-negative, placental cell lines, in the presence or absence of soluble CD4. Env sequence analysis revealed several differences among viruses with different tropisms, most notably a four amino acid deletion in the central region of the V3 loop that distinguishes the R5 virus P6-v3-25-4 from the R5, Bonzo virus P6-v3-22-1.

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Documento generato il 04/12/20 alle ore 06:52:28