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Titolo:
PILOT-SCALE PURIFICATION OF HUMAN MONOCLONAL IGM (COU-1) FOR CLINICAL-TRIALS
Autore:
TORNOE I; TITLESTAD IL; KEJLING K; ERB K; DITZEL HJ; JENSENIUS JC;
Indirizzi:
ODENSE UNIV,BIOMED LAB,WINSLOWPARKEN 21 DK-5000 ODENSE C DENMARK ODENSE UNIV,BIOMED LAB DK-5000 ODENSE C DENMARK ODENSE UNIV,DEPT MICROBIOL ODENSE DENMARK COPENHAGEN UNIV HOSP,DEPT CLIN IMMUNOL,RIGSHOSP COPENHAGEN DENMARK UNIV AARHUS,INST MED MICROBIOL & IMMUNOL AARHUS DENMARK
Titolo Testata:
Journal of immunological methods
fascicolo: 1, volume: 205, anno: 1997,
pagine: 11 - 17
SICI:
0022-1759(1997)205:1<11:PPOHMI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROXYLAPATITE CHROMATOGRAPHY; TUMOR-LOCALIZATION; COLORECTAL-CANCER; ANTIBODY COU-1;
Keywords:
HUMAN MONOCLONAL ANTIBODY; IGM PURIFICATION; PILOT SCALE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
I. Tornoe et al., "PILOT-SCALE PURIFICATION OF HUMAN MONOCLONAL IGM (COU-1) FOR CLINICAL-TRIALS", Journal of immunological methods, 205(1), 1997, pp. 11-17

Abstract

No standard procedure is available for the purification of human monoclonal antibodies for human i.v. administration, Here we describe the procedure developed for pilot scale purification of the human IgM monoclonal antibody COU-1 directed against a cancer-associated antigen. The hybridoma cells were grown in protein-free medium and purification from the clarified culture supernatant was carried out in 4 simple chromatographic steps: (1) hydroxylapatite chromatography; (2) hydrophobicinteraction chromatography on phenyl-Sepharose: (3) cation-exchange chromatography on sulphonyl-Sepharose; and (4) anion-exchange chromatography on tetraethylamino-Sepharose. The product was substantially purewith regard to protein after step 3, but contained DNA which was removed in step 4. The average recovery of the IgM was 54% with a range of40-65%. Importantly, the ability of the antibody to bind to its antigen in ELISA was fully maintained during the purification. Subsequently, the purified antibody was isotope labelled and successfully used forin vivo detection of colon, rectal and pancreas carcinomas in patients. The purification procedure described appears to compare favourably with previously published methods, but a critical comparison is not possible due to the lack of necessary information in the available literature.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 12:04:36