Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization of the ecdysteroid UDP-Glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus
Autore:
Rodrigues, JCM; De Souza, ML; OReilly, D; Velloso, LM; Pinedo, FJR; Razuck, FB; Ribeiro, B; Ribeiro, BM;
Indirizzi:
Univ Brasilia, Dept Cell Biol, BR-70919970 Brasilia, DF, Brazil Univ Brasilia Brasilia DF Brazil BR-70919970 BC19970 Brasilia, DF, Brazil Embrapa Genet Resources & Biotechnol, Brasilia, DF, Brazil Embrapa Genet Resources & Biotechnol Brasilia DF Brazil ilia, DF, Brazil Univ London Imperial Coll Sci Technol & Med, Dept Biol, London, England Univ London Imperial Coll Sci Technol & Med London England don, England
Titolo Testata:
VIRUS GENES
fascicolo: 1, volume: 22, anno: 2001,
pagine: 103 - 112
SICI:
0920-8569(200101)22:1<103:COTEU(>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR POLYHEDROSIS-VIRUS; CELL-LINES; GLUCOSYL TRANSFERASE; SEQUENCE-ANALYSIS; EXPRESSION; BACULOVIRUSES; DNA; IDENTIFICATION; TRANSCRIPTION; REPLICATION;
Keywords:
baculovirus; Anticarsia gemmatalis; AgMNPV; insect cells; EGT; nucleopolyhedrovirus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Ribeiro, BM Univ Brasilia, Dept Cell Biol, BR-70919970 Brasilia, DF, Brazil Univ Brasilia Brasilia DF Brazil BR-70919970 BCia, DF, Brazil
Citazione:
J.C.M. Rodrigues et al., "Characterization of the ecdysteroid UDP-Glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus", VIRUS GENES, 22(1), 2001, pp. 103-112

Abstract

The Anticarsia gemmatalis nucelopolyhedrovirus (AgMNPV) egt gene was cloned, sequenced and its expression characterized by RT-PCR and western blot analysis. Sequence analysis of the gene indicated the presence of an open reading frame (ORF) of 1482 nucleotides, which codes for a polypeptide of 494 amino acids. A TATA box and a conserved regulatory sequence (CATT) found inother baculovirus early genes were present in the promoter region of the egt gene. A poly-A consensus sequence was present in the 3' untranslated region (3'-UTR) of the gene. Homology comparisons showed that the EGT protein of AgMNPV is most closely related (95.9% amino acid sequence identity) to the EGT from the Choristoneura fumiferana DEF nucleopolyhedrovirus (CfDEF). Transcriptional analysis of the AgMNPV egt gene showed that egt-specific transcripts can be detected both early and late in infection. The EGT proteinwas detected, by western blot analysis, in the intra- (from 12 to 48 h post-infection) and extra-cellular (from 12 to 96 h post-infection) fractions of infected insect cells. The AgMNPV Bgl II-F fragment, which has homology to the AcMNPV ie-1 gene, was cloned and used to cotransfect SF21 cells withthe cloned AgMNPV egt gene. EGT activity was observed, suggesting that AgMNPV ie-1 can transactivate egt expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:27:07