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Titolo:
Antigenic and molecular analyses reveal that the equine rotavirus strain H-1 is closely related to porcine, but not equine, rotaviruses: Interspeciestransmission from pigs to horses?
Autore:
Ciarlet, M; Isa, P; Conner, ME; Liprandi, F;
Indirizzi:
Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 l & Microbiol, Houston, TX 77030 USA Moredun Res Inst, Edinburgh EH17 7JH, Midlothian, Scotland Moredun Res Inst Edinburgh Midlothian Scotland EH17 7JH lothian, Scotland Vet Affairs Med Ctr, Houston, TX 77030 USA Vet Affairs Med Ctr Houston TXUSA 77030 s Med Ctr, Houston, TX 77030 USA Inst Venezolano Invest Cient, Ctr Microbiol & Biol Celular, Caracas 1020A,Venezuela Inst Venezolano Invest Cient Caracas Venezuela 1020A cas 1020A,Venezuela
Titolo Testata:
VIRUS GENES
fascicolo: 1, volume: 22, anno: 2001,
pagine: 5 - 20
SICI:
0920-8569(200101)22:1<5:AAMART>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEROTYPE-SPECIFIC GLYCOPROTEIN; COMPARATIVE SEQUENCE-ANALYSIS; NUCLEOTIDE-SEQUENCE; GENOMIC CHARACTERIZATION; NEUTRALIZATION EPITOPES; BOVINE ROTAVIRUS; CAPSID PROTEIN; GENE SEQUENCE; NONSTRUCTURAL GLYCOPROTEIN; CODING ASSIGNMENT;
Keywords:
rotavirus; interspecies transmission; pigs; horses; nucleotide sequence; pathogenicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Ciarlet, M Baylor Coll Med, Dept Mol Virol & Microbiol, 1 Baylor Plaza,Room 921E,Mailstop BCM-385, Houston, TX 77030 USA Baylor Coll Med 1 Baylor Plaza,Room 921E,Mailstop BCM-385 Houston TX USA 77030
Citazione:
M. Ciarlet et al., "Antigenic and molecular analyses reveal that the equine rotavirus strain H-1 is closely related to porcine, but not equine, rotaviruses: Interspeciestransmission from pigs to horses?", VIRUS GENES, 22(1), 2001, pp. 5-20

Abstract

We have sequenced the genes encoding the inner capsid protein VP6 and the outer capsid glycoprotein VP7 of the subgroup (SG) I equine rotavirus strain H-1 (P9[7], G5). The VP6 and VP7 proteins of the equine rotavirus strain H-1 shared a high degree of sequence and deduced amino acid identity with SG I porcine strains and serotype G5 porcine strains, respectively. Previoussequence analyses of the genes encoding the outer capsid spike protein VP4and the nonstructural proteins NSP1 and NSP4 of equine H-1 strain also revealed a high degree of sequence and deduced amino acid homology with the prototype porcine rotavirus strain OSU (P9[7], G5). We have also confirmed and extended the VP4 and VP7 antigenic relatedness of equine rotavirus strainH-1 to porcine strains of P9[7] and G5 serotype specificities isolated in the United States, Venezuela, Argentina, and Australia based on cross-neutralization studies. In addition, the pathogenicity of tissue culture-adaptedequine H-1, H-2, FI-14, FI-23, and L338, and porcine OSU rotavirus strainswas compared in the neonatal mouse model. The 50% diarrhea dose (DD50) of equine H-1 was similar to that of porcine OSU and equine H-2 and L338 strains, while the DD50 of equine H-2 was greater than or equal to 50 or 315-fold lower than those of equine FI-14 or FI-23, respectively. Our sequence comparison of NSP4 of the rotavirus strains tested potentially identified amino acid residue 136, within the variable region spanning amino acids 130 to 141, as playing a role in virulence. Taken together, there is strong support to suggest that the equine rotavirus strain H-1 may represent an example of interspecies transmission from pigs to horses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 16:37:47