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Titolo:
Structure of the maize streak virus geminate particle
Autore:
Zhang, W; Olson, NH; Baker, TS; Faulkner, L; Agbandje-McKenna, M; Boulton, MI; Davies, JW; McKenna, R;
Indirizzi:
Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 Biol Sci, W Lafayette, IN 47907 USA Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England Univ Warwick Coventry W Midlands England CV4 7AL 7AL, W Midlands, England Univ Florida, Inst Brain, Coll Med, Dept Biochem & Mol Biol, Gainesville, FL 32610 USA Univ Florida Gainesville FL USA 32610 Mol Biol, Gainesville, FL 32610 USA John Innes Inst, Dept Virus Res, Norwich NR4 7UH, Norfolk, England John Innes Inst Norwich Norfolk England NR4 7UH NR4 7UH, Norfolk, England
Titolo Testata:
VIROLOGY
fascicolo: 2, volume: 279, anno: 2001,
pagine: 471 - 477
SICI:
0042-6822(20010120)279:2<471:SOTMSV>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUNCTIONAL IMPLICATIONS; 3-DIMENSIONAL RECONSTRUCTION; BIOLOGICAL MACROMOLECULES; MOLECULAR-BIOLOGY; ATOMIC-STRUCTURE; DNA; PROTEIN; PARVOVIRUS; SEQUENCE; GEMINIVIRUSES;
Keywords:
cryo-electron microscopy; Geminiviridae; icosahedral capsid; maize streak virus; ssDNA virus; three-dimensional image reconstruction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: McKenna, R Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 W Lafayette, IN 47907 USA
Citazione:
W. Zhang et al., "Structure of the maize streak virus geminate particle", VIROLOGY, 279(2), 2001, pp. 471-477

Abstract

lThe Geminiviridae is an extensive family of plant viruses responsible foreconomically devastating diseases in crops worldwide. Geminiviruses package circular, single-stranded DNA (ssDNA) genomes. The characteristic twinnedor "geminate" particles, which consist of two joined, incomplete T = 1 icosahedra, are unique among viruses. We have determined the first structure of a geminivirus particle, the Nigerian strain of Maize streak virus (MSV-N), using cryo-electron microscopy and three-dimensional image reconstructionmethods. The particle, of dimensions 220 x 380 Angstrom, has an overall 52-point-group symmetry, in which each half particle "head" consists of the coal protein (CP) arranged with quasi-icosahedral symmetry. We have modeled the MSV-N CP as an eight-stranded, antiparallel beta -barrel motif (a structural motif common to all known ssDNA viruses) with an N-terminal alpha -helix. This has produced a model of the geminate particle in which 110 copiesof the CP nicely fit into the reconstructed density map. The reconstructeddensity map and MSV-N pseudo-atomic model demonstrate that the geminate particle has a stable, defined structure. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 02:05:07