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Titolo:
The structure of isometric capsids of bacteriophage T4
Autore:
Olson, NH; Gingery, M; Eiserling, FA; Baker, TS;
Indirizzi:
Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 Biol Sci, W Lafayette, IN 47907 USA Univ Calif Los Angeles, Dept Microbiol & Mol Genet, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
VIROLOGY
fascicolo: 2, volume: 279, anno: 2001,
pagine: 385 - 391
SICI:
0042-6822(20010120)279:2<385:TSOICO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-ORGANIZATION; SURFACE LATTICE; DNA; HEAD; PROTEINS; VIRUS; SHELL; MODEL;
Keywords:
bacteriophage T4; cryoelectron microscopy; three-dimensional image reconstruction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Baker, TS Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 W Lafayette, IN 47907 USA
Citazione:
N.H. Olson et al., "The structure of isometric capsids of bacteriophage T4", VIROLOGY, 279(2), 2001, pp. 385-391

Abstract

three-dimensional structure of DNA-filled, bacteriophage T4 isometric capsids has been determined by means of cryoelectron microscopy and image reconstruction techniques. The packing geometry of protein subunits on the capsid surface was confirmed to be that of the triangulation class T = 13. The reconstruction clearly shows pentamers, attributed to capsid protein gp24*, surrounded by hexamers of the major capsid protein, gp23*. Positions of theaccessory proteins, Hoc and Sec, are also clearly delineated in the surface lattice. The Hoc protein is the most prominent surface feature and appears as an extended molecule with a rounded base from which a thin neck and a globular head protrude. One Hoc molecule associates with each hexamer. Nearly continuous "ridges" are formed at the periphery of the gp23* hexamers byan association of 12 Soc molecules; however, Soc is absent along the boundaries between the hexamers and the pentamers. The duplex DNA genome forms ahighly condensed series of concentric layers, spaced about 2.36 nm apart, that follow the general contour of the inner wall of the protein capsid. (C) 2001 Academic Press.

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Documento generato il 28/01/20 alle ore 21:04:05