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Titolo:
Sensitive and rapid method for the simultaneous quantification of the HIV-protease inhibitors indinavir, nelfinavir, ritonavir, and saquinavir in human plasma by reversed-phase liquid chromatography
Autore:
Bouley, M; Briere, C; Padoin, C; Petitjean, O; Tod, M;
Indirizzi:
Univ Bobigny, Hop Avicenne, Dept Pharmacotoxicol, F-93009 Bobigny, France Univ Bobigny Bobigny France F-93009 macotoxicol, F-93009 Bobigny, France
Titolo Testata:
THERAPEUTIC DRUG MONITORING
fascicolo: 1, volume: 23, anno: 2001,
pagine: 56 - 60
SICI:
0163-4356(200102)23:1<56:SARMFT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ULTRAVIOLET DETECTION;
Keywords:
liquid chromatography; indinavir; nelfinavir; ritonavir; saquinavir;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Tod, M Univ Bobigny, Hop Avicenne, Dept Pharmacotoxicol, 125 Route Stalingrad, F-93009 Bobigny, France Univ Bobigny 125 Route Stalingrad Bobigny France F-93009 y, France
Citazione:
M. Bouley et al., "Sensitive and rapid method for the simultaneous quantification of the HIV-protease inhibitors indinavir, nelfinavir, ritonavir, and saquinavir in human plasma by reversed-phase liquid chromatography", THER DRUG M, 23(1), 2001, pp. 56-60

Abstract

A rapid, sensitive, and specific liquid chromatography method for the simultaneous determination of four protease inhibitors (indinavir, nelfinavir, ritonavir, and saquinavir) in human plasma is described. After a liquid-liquid extraction with terbutyl methyl ether and a sequential washing of the reconstituted sample with hexane, protease inhibitors are separated on a phenyl column using a simple binary mobile phase of ammonium acetate buffer:acetonitrile (48:52) (pH = 7.5) with an ultraviolet detection at 260 nm. The standard curves are linear in the range 0.025-1 mug/mL for saquinavir. 0.1-4 mug/mL for indinavir and nelfinavir, and 0.25-10 mug/mL for ritonavir, with an average recovery ranging from 79% to 99%, and with both low interday and intraday coefficients of variation (<15%). This assay is simple, rapid (15-minute interval between runs). and useful for therapeutic monitoring ofthe protease inhibitors on a routine basis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 09:32:28