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Titolo:
Quantification of D-1B (D-5) receptors in dopamine D-1A receptor-deficientmice
Autore:
Montague, DM; Striplin, CD; Overcash, JS; Drago, J; Lawler, CP; Mailman, RB;
Indirizzi:
Univ N Carolina, Sch Med, Ctr Neurosci, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 rosci, Chapel Hill, NC 27599 USA Univ N Carolina, Sch Med, Dept Pharmacol, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA , Dept Pharmacol, Chapel Hill, NC USA Univ N Carolina, Sch Med, Dept Psychiat, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA d, Dept Psychiat, Chapel Hill, NC USA Univ N Carolina, Sch Med, Curriculum Toxicol, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA rriculum Toxicol, Chapel Hill, NC USA Monash Univ, Dept Anat, Neurosci Unit, Clayton, Vic 3168, Australia MonashUniv Clayton Vic Australia 3168 Unit, Clayton, Vic 3168, Australia
Titolo Testata:
SYNAPSE
fascicolo: 4, volume: 39, anno: 2001,
pagine: 319 - 322
SICI:
0887-4476(20010315)39:4<319:QOD(RI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-BRAIN; PHOSPHOINOSITIDE HYDROLYSIS; CHOLINERGIC INTERNEURONS; D1; EXPRESSION; GENE; CLONING; ACTIVATION; STIMULATION; SCH23390;
Keywords:
D-1 receptors; D-1A receptors; D-1B receptors; D-5 receptors; dopamine; receptor autoradiography;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Mailman, RB Univ N Carolina, Sch Med, Ctr Neurosci, CB 7250, Chapel Hill, NC 27599 USA Univ N Carolina CB 7250 Chapel Hill NC USA 27599 NC 27599 USA
Citazione:
D.M. Montague et al., "Quantification of D-1B (D-5) receptors in dopamine D-1A receptor-deficientmice", SYNAPSE, 39(4), 2001, pp. 319-322

Abstract

The unavailability of selective D-1A (D-1) or D-1B (D-5) dopamine receptorligands has prevented the direct localization of binding sites for these receptors. Thus, receptor autoradiography with long exposure times was used to detect minor D-1-like binding sites in the brains of D-1A null mutants. Coronal brain sections were prepared from the caudal portion of the prefrontal cortex of homozygous or heterozygous D-1A knockout mice or wildtype mice, and labeled with the D-1 receptor antagonist [H-3]- SCH23390. Slides were dried, and apposed to film with polymer-calibrated standards for 90 days to allow visualization of any low abundance binding sites. No binding was detected in most regions of homozygote (-/-) mouse brains that have high densities of D-1 binding in wildtype mice (e.g., the striatum, nucleus accumbens, olfactory tubercles or amygdala). Conversely, small, but detectable amounts of D-1-binding were measured in the hippocampus, albeit with a densityless than the lowest standard (ca. 20 fmol/mg). Saturation binding of [H-3]-SCH23390 in hippocampal homogenates from homozygous mice confirmed a B-max of 12.3 fmol/mg protein with a K-D of 0.57 nM. The current work demonstrates directly the presence of D-1B (D-5) receptors in hippocampus, and also shows that; the loss of functional D-1A gene products almost completely eliminates detectable D-1-binding sites in striatum, as well as in some regions (e.g., the amygdala) where a non-adenylyl cyclase coupled D-1 receptor has been reported. This indicates that these non-adenylyl cyclase coupled D-1-like receptors represent alternate signaling pathways rather than novel gene products(s). Synapse 39:319-322, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 01:14:06