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Titolo:
The decrease of PKC alpha is associated with hepatic apoptosis at early and late phases of polymicrobial sepsis
Autore:
Jao, HC; Yang, RC; Hsu, HK; Hsu, C;
Indirizzi:
Kaohsiung Med Coll, Dept Physiol, Kaohsiung, Taiwan Kaohsiung Med Coll Kaohsiung Taiwan ll, Dept Physiol, Kaohsiung, Taiwan
Titolo Testata:
SHOCK
fascicolo: 2, volume: 15, anno: 2001,
pagine: 130 - 134
SICI:
1073-2322(200102)15:2<130:TDOPAI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; CELL-DEATH; RAT-LIVER; BCL-2; PHOSPHORYLATION; INHIBITION; SHOCK;
Keywords:
hepatocytes; apoptosis; PKC alpha; Bcl-2; Bcl-xL; Bax; PARP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Hsu, C Kaohsiung Med Univ, Dept Physiol, 100 Shih Chuan 1st Rd, Kaohsiung 807, Taiwan Kaohsiung Med Univ 100 Shih Chuan 1st Rd Kaohsiung Taiwan 807 iwan
Citazione:
H.C. Jao et al., "The decrease of PKC alpha is associated with hepatic apoptosis at early and late phases of polymicrobial sepsis", SHOCK, 15(2), 2001, pp. 130-134

Abstract

The present study investigates the relationship between the PKC-alpha and hepatic apoptosis during sepsis. Cecal ligation and puncture- (CLP) inducedanimal model of polymicrobial sepsis was used, with early and late sepsis referring to those animals sacrificed at 9 and 18 h, respectively, after CLP, The expressions of PKC alpha and Bcl-2 family proteins as well as poly(ADP-ribose) polymerase (PARP) cleavage were quantified to evaluate the possible factors involved in the hepatic cell death during sepsis. The apoptosisof hepatocytes under septic condition or hepatocytes treated with PKC alpha antisense was evaluated by gel electrophoresis and/or flow cytometry after Annexin-V-Fluos and propidium iodide staining. The results indicated that(1) the protein expression of membrane-associated PKC alpha was decreased at early (P < 0.05) and late (P < 0.01) sepsis; (2) the protein expressionsof Bcl-2 and Bcl-xL were decreased, whereas Bax expression was increased at late sepsis; (3) the percentage of PARP cleavage was increased at early (P < 0.05) and late (P < 0.01) sepsis; (4) severe DNA fragmentation was observed at late sepsis; (5) the apoptotic cell population was increased at early and late sepsis; and (6) the percentage of apoptotic cell population in PKC<alpha> antisense-treated cells was significantly higher than that in untreated cells. These results suggest that inactivation of PKC alpha may play an important role in modulating hepatic apoptosis during sepsis and the apoptosis is closely associated with the alterations of Bcl-2 family proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:46:54