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Titolo:
Endogenous hormone levels in habituated nucellar Citrus callus during the initial stages of regeneration
Autore:
Jimenez, VM; Guevara, E; Herrera, J; Bangerth, F;
Indirizzi:
Univ Hohenheim, Inst Obst Gemuse & Weinbau 370, D-70593 Stuttgart, GermanyUniv Hohenheim Stuttgart Germany D-70593 370, D-70593 Stuttgart, Germany Univ Costa Rica, CIGRAS, San Pedro 2060, Costa Rica Univ Costa Rica San Pedro Costa Rica 2060 AS, San Pedro 2060, Costa Rica
Titolo Testata:
PLANT CELL REPORTS
fascicolo: 1, volume: 20, anno: 2001,
pagine: 92 - 100
SICI:
0721-7714(200101)20:1<92:EHLIHN>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PIMPINELLA-ANISUM L; SOMATIC EMBRYOGENESIS; OVULAR CALLUS; ABSCISIC-ACID; CELL-CULTURES; EMBRYOID DEVELOPMENT; GLYCEROL; ORANGE; CARROT; STIMULATION;
Keywords:
phytohormones; somatic embryogenesis; glycerol; sucrose; in vitro culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Bangerth, F Univ Hohenheim, Inst Obst Gemuse & Weinbau 370, D-70593 Stuttgart, Germany Univ Hohenheim Stuttgart Germany D-70593 Stuttgart, Germany
Citazione:
V.M. Jimenez et al., "Endogenous hormone levels in habituated nucellar Citrus callus during the initial stages of regeneration", PL CELL REP, 20(1), 2001, pp. 92-100

Abstract

Embryogenic nucellar callus cultures of different Citrus species and cultivars growing in hormone-free medium were transferred to medium containing either sucrose or glycerol as the only carbohydrate source. Glycerol has been reported to induce further development of Citrus somatic embryos, while in the presence of sucrose they continue to proliferate in an 'undifferentiated' manner. The endogenous hormone levels of the cultures were evaluated after 2 and 5 days to characterise the initial steps of embryo development. In most cases, differences among treatments were observed only after 5 daysof culture. Higher cytokinin levels were found in most of the cultures transferred to the glycerol-containing medium. The effect of ageing sweet orange cultures on their endogenous hormone levels was determined by leaving them in the original culture medium without subculturing for 60 days. While no changes were observed in the free indoleacetic acid and gibberellin contents. lower levels of abscisic acid and cytokinins were found in the aged cultures than in those transferred at the normal interval. every 30 days. Theendogenous hormone contents of Citrus callus of different genotypes were compared. Significant differences were observed in the levels of all hormones evaluated, even when the in vitro ontogeny of the different genotypes wasvery similar.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 21:59:07