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Titolo:
Overexpression of the AtGluR2 gene encoding an arabidopsis homolog of mammalian glutamate receptors impairs calcium utilization and sensitivity to ionic stress in transgenic plants
Autore:
Kim, SA; Kwak, JM; Jae, SK; Wang, MH; Nam, HG;
Indirizzi:
Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, Kyungbuk, South Korea Pohang Univ Sci & Technol Pohang Kyungbuk South Korea 790784 South Korea Univ Calif San Diego, Cell & Dev Biol Sect, Div Biol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 v Biol, La Jolla, CA 92093 USA Univ Calif San Diego, Ctr Mol Genet, La Jolla, CA 92093 USA Univ Calif SanDiego La Jolla CA USA 92093 Genet, La Jolla, CA 92093 USA
Titolo Testata:
PLANT AND CELL PHYSIOLOGY
fascicolo: 1, volume: 42, anno: 2001,
pagine: 74 - 84
SICI:
0032-0781(200101)42:1<74:OOTAGE>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUNCTIONAL-CHARACTERIZATION; CA2+ PERMEABILITY; CHANNELS; CELLS; EXPRESSION; CLONING; SUBUNIT; SITE; HOMEOSTASIS; EFFICIENCY;
Keywords:
Arabidopsis thaliana; calcium efficiency; critical deficiency content; glutamate receptor homolog; ion hypersensitivity; xylem unloading;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Nam, HG Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, Kyungbuk, South Korea Pohang Univ Sci & Technol Pohang Kyungbuk South Korea 790784 orea
Citazione:
S.A. Kim et al., "Overexpression of the AtGluR2 gene encoding an arabidopsis homolog of mammalian glutamate receptors impairs calcium utilization and sensitivity to ionic stress in transgenic plants", PLANT CEL P, 42(1), 2001, pp. 74-84

Abstract

We have identified a homolog of the mammalian iono-tropic glutamate receptor genes in Arabidopsis thaliana (AtGluR2). This gene was found to alter Ca2+ utilization when overexpressed in A. thaliana. These transgenic plants displayed symptoms of Ca2+ deficiency, including browning and death of the shoot apex, necrosis of leaf tips, and deformation of leaves. Supplementation with Ca2+ alleviated these phenotypes. Overall levels of Ca2+ in tissues of control plants were not significantly different from those of transgenicplants, suggesting that overexpression of the AtGluR2 gene did not affect Ca2+ uptake. However, the relative growth yield as a function of Ca2+ levels revealed that the critical deficiency content of Ca2+ in transgenic plants was three times higher than that of control plants. The transgenic plantsalso exhibited hypersensitivity to Na+ and K+ ionic stresses. The ion hypersensitivity was ameliorated by supplementation with Ca2+. The results showed that overexpression of the AtGluR2 gene caused reduced efficiency of Ca2 utilization in the transgenic plants. The promoter of the AtGluR2 gene was active in vascular tissues, particularly in cells adjacent to the conducting vessels. This suggests that AtGluR2 encodes a functional channel that unloads Ca2+ from the xylem vessels. The results together suggest that appropriate expression of the AtGluR2 protein may play critical roles in Ca2+ nutrition by controlling the ion allocation among different Ca2+ sinks both during normal development and during adaptation to ionic stresses.

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Documento generato il 02/04/20 alle ore 01:31:03