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Titolo:
Pumpkin peroxisomal ascorbate peroxidase is localized on peroxisomal membranes and unknown membranous structures
Autore:
Nito, K; Yamaguchi, K; Kondo, M; Hayashi, M; Nishimura, M;
Indirizzi:
Natl Inst Basic Biol, Dept Cell Biol, Okazaki, Aichi 4448585, Japan Natl Inst Basic Biol Okazaki Aichi Japan 4448585 ki, Aichi 4448585, Japan Grad Univ Adv Studies, Sch Life Sci, Dept Mol Biomech, Okazaki, Aichi 4448585, Japan Grad Univ Adv Studies Okazaki Aichi Japan 4448585 i, Aichi 4448585, Japan
Titolo Testata:
PLANT AND CELL PHYSIOLOGY
fascicolo: 1, volume: 42, anno: 2001,
pagine: 20 - 27
SICI:
0032-0781(200101)42:1<20:PPAPIL>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYOXYSOMAL CITRATE SYNTHASE; TERMINAL TARGETING SIGNAL; HIGHER MOLECULAR-MASS; ACYL-COA OXIDASE; LEAF PEROXISOMES; PROTEIN IMPORT; CDNA CLONING; HYDROXYPYRUVATE REDUCTASE; AGROBACTERIUM-TUMEFACIENS; ENDOPLASMIC-RETICULUM;
Keywords:
ascorbate peroxidase; endoplasmic reticulum; hydrogen peroxide; peroxisome; protein transport; pumpkin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Nishimura, M Natl Inst Basic Biol, Dept Cell Biol, Okazaki, Aichi 4448585,Japan Natl Inst Basic Biol Okazaki Aichi Japan 4448585 8585, Japan
Citazione:
K. Nito et al., "Pumpkin peroxisomal ascorbate peroxidase is localized on peroxisomal membranes and unknown membranous structures", PLANT CEL P, 42(1), 2001, pp. 20-27

Abstract

To investigate the roles of peroxisomal membrane proteins in the reversible conversion of glyoxysomes to leaf peroxisomes, we characterized several membrane proteins of glyoxysomes, One of them was identified as an ascorbateperoxidase (pAPX) that is localized on glyoxysomal membranes. Its cDNA wasisolated by immunoscreening, The deduced amino acid sequence encoded by the cDNA insert does not have a peroxisomal targeting signal (PTS), suggesting that pAPX is imported by one or more PTS-independent pathways. Subcellular fractionation of 3- and 5-d-old cotyledons of pumpkin revealed that pAPX was localized not only in the glyoxysomal fraction, but also in the ER fraction. A magnesium shift experiment showed that the density of pAPX in the ER fraction did not increase in the presence of Mg2+, indicating that pAPX is not localized in the rough ER, Immunocytochemical analysis using a transgenic Arabidopsis which expressed pumpkin pAPX showed that pAPX was localized on peroxisomal membranes, and also on a unknown membranous structure in green cotyledons, The overall results suggested that pAPX is transported to glyoxysomal membranes via this unknown membranous structure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 22:52:48