Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Mutagenesis in eukaryotes dependent on DNA polymerase zeta and Rev1p
Autore:
Lawrence, CW; Maher, VM;
Indirizzi:
Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 & Biophys, Rochester, NY 14642 USA Michigan State Univ, Dept Microbiol, Carcinogenesis Lab, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 s Lab, E Lansing, MI 48824 USA Michigan State Univ, Dept Biochem, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 ochem, E Lansing, MI 48824 USA
Titolo Testata:
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
fascicolo: 1405, volume: 356, anno: 2001,
pagine: 41 - 46
SICI:
0962-8436(20010129)356:1405<41:MIEDOD>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
DAMAGE-INDUCED MUTAGENESIS; ESCHERICHIA-COLI DINB; THYMINE-THYMINE DIMER; SACCHAROMYCES-CEREVISIAE; SPONTANEOUS MUTATION; EXCISION-REPAIR; ABASIC SITES; YEAST; GENE; UV;
Keywords:
DNA polymerase zeta; mutagenesis; Rev1p; yeast; human cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Lawrence, CW Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 ochester, NY 14642 USA
Citazione:
C.W. Lawrence e V.M. Maher, "Mutagenesis in eukaryotes dependent on DNA polymerase zeta and Rev1p", PHI T ROY B, 356(1405), 2001, pp. 41-46

Abstract

DNA polymerase zeta (Pol zeta) and Rev1p carry out translesion replicationin budding yeast, Saccharomyces cerevisiae, and are jointly responsible for almost all base pair substitution and frameshift mutations induced by DNAdamage in this organism. In addition, Pol zeta is responsible for the majority of spontaneous mutations in yeast and has been proposed as the enzyme responsible for somatic hypermutability. Pol zeta, a non-processive enzyme that lacks a 3' to 5' exonuclease proofreading activity, is composed of Rev3p, the catalytic subunit, and a second subunit encoded by REV7. In keepingwith its role, extension by Pol zeta is relatively tolerant of abnormal DNA structure at the primer terminus and is much more capable of extension from terminal mismatches than yeast DNA polymerase alpha (Pol alpha). Rev1p is a bifunctional enzyme that possesses a deoxycytidyl transferase activity that incorporates deoxycytidyl opposite abasic sites in the template and a second, at present poorly defined, activity that is required for the bypassof a variety of lesions as well as abasic sites. Human homologues of the yeast REV1 and REV3 have been identified and, based on the phenotype of cells producing antisense RNA to one or other of these genes, their products appear also to be employed in translation replication and spontaneous mutagenesis. We suggest that Pol zeta is best regarded as a replication enzyme, albeit one that is used only intermittently, that promotes extension at forksthe progress of which is blocked for any reason, whether the presence of an unedited terminal mismatch or unrepaired DNA lesion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 12:41:39