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Titolo:
PRELIMINARY FINDINGS OF AN IN-VITRO HUMAN SPLEEN MONONUCLEAR CELL-CULTURE SYSTEM FOR PRIMARY ISOLATES OF HIV TYPE-1
Autore:
TSAI WP; RIMELZWAAN GF; MERGES MJ; WU SC; CONLEY S; KUNG HF; GARRITY R; GOUDSMIT J; NARA PL;
Indirizzi:
NCI,FREDERICK CANC RES & DEV CTR,LAB BIOCHEM PHYSIOL,DIV BASIC SCI,POB B,BLDG 567,RM 182 FREDERICK MD 21702 NCI,FREDERICK CANC RES & DEV CTR,VACCINE RESISTANT DIS SECT,DIRECTORSLAB,OFF DIRECTOR FREDERICK MD 21702 NCI,FREDERICK CANC RES & DEV CTR,LAB BIOCHEM PHYSIOL,DIV BASIC SCI FREDERICK MD 21702 ACAD MED CTR,HUMAN RETROVIRUS LAB,DEPT VIROL AMSTERDAM NETHERLANDS NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT BETHESDA MD 20894 SCI APPLICAT INT CORP FREDERICK MD 21702
Titolo Testata:
AIDS research and human retroviruses
fascicolo: 11, volume: 13, anno: 1997,
pagine: 967 - 977
SICI:
0889-2229(1997)13:11<967:PFOAIH>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HUMAN KUPFFER CELLS; CAPILLARY ENDOTHELIAL-CELLS; POLYMERASE CHAIN-REACTION; MESSENGER-RNA EXPRESSION; INFECTIOUS-ANEMIA VIRUS; T-CELLS; RHESUS MACAQUES; VIRAL BURDEN; DISEASE PROGRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
78
Recensione:
Indirizzi per estratti:
Citazione:
W.P. Tsai et al., "PRELIMINARY FINDINGS OF AN IN-VITRO HUMAN SPLEEN MONONUCLEAR CELL-CULTURE SYSTEM FOR PRIMARY ISOLATES OF HIV TYPE-1", AIDS research and human retroviruses, 13(11), 1997, pp. 967-977

Abstract

Acute HIV-1 infection is often manifested with a high level of viremia, The cell types and tissues/organs that contribute to the virus loadare thought to be of central and peripheral lymphoreticular origin, The establishment and permissiveness of organ-based cell culture systems from spleen with laboratory strains or primary isolates of HIV-1 have not been reported, We studied unseparated splenic mononuclear cells (SMCs) and adherent cells derived from human spleen and liver in comparison with blood monocyte-derived macrophages (MDMs), Unstimulated, SMCs were highly permissive to primary lymphotropic HIV-1 and dual/macrophage-tropic isolates (which are able to replicate in both MDMs and PBMCs). Furthermore, SMCs were found to replicate virus to high titer ina rapid log-phase manner and exhibited a prolonged stationary phase of virus production, unlike PBMCs, which required conventional activation with mitogens and exhibited a much shorter period of virus production, Interestingly, the SMCs maintained themselves as a mixed phenotypeof nested lymphocytes with complex and well-differentiated macrophage(s) for extended periods of time, In addition, splenic macrophages readily purified by adherence were highly permissive to a dual/macrophage-tropic primary isolate, HIV-1(ADA), intermediate with two laboratory strains, HIV-1(RF) and HIV-1(HXB3), and least permissive to the lymphotropic primary isolate HIV-1(Mr452) and two other laboratory strains, HIV-1(CC) and HIV-1(MN). The replication of HIV-1(ADA) as measured by extracellular p24 was sustained for up to 7 weeks and similar to the replication patterns observed with adherent hepatic macrophages and blood-derived MDMs, This study demonstrates that exogenous stimulation isnot required for infection of these cells; either adherence-isolated and/or mixed lymphoid populations can be studied together, and viable stocks can be readily prepared and cryopreserved, In addition, these cells could be used for isolating new and/or other variants of HIV-1. Thus, the use of the SMC primary in vitro cell culture system for future studies involving HIV-1 is warranted.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/02/20 alle ore 14:53:18