Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The protein-protein interaction map of Helicobacter pylori (vol 409, pg 211, 2001)
Autore:
Rain, JC; Selig, L; De Reuse, H; Battaglia, V; Reverdy, C; Simon, S; Lenzen, G; Petel, F; Wojcik, J; Schachter, V; Chemama, Y; Labigne, AS; Legrain, P;
Indirizzi:
Hybrigen SA, F-75012 Paris, France Hybrigen SA Paris France F-75012Hybrigen SA, F-75012 Paris, France Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, France Inst Pasteur Paris France 15 erienne Muqueuses, F-75724 Paris 15, France
Titolo Testata:
NATURE
fascicolo: 6821, volume: 409, anno: 2001,
pagine: 743 -
SICI:
0028-0836(20010208)409:6821<743:TPIMOH>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; COMPLETE GENOME; RNA-POLYMERASE; SEQUENCE; YEAST;
Tipo documento:
Correction, Addition
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Rain, JC Hybrigen SA, 180 Ave Daumesnil, F-75012 Paris, France Hybrigen SA180 Ave Daumesnil Paris France F-75012 Paris, France
Citazione:
J.C. Rain et al., "The protein-protein interaction map of Helicobacter pylori (vol 409, pg 211, 2001)", NATURE, 409(6821), 2001, pp. 743

Abstract

With the availability of complete DNA sequences for many prokaryotic and eukaryotic genomes, and soon for the human genome itself, it is important todevelop reliable proteome-wide approaches for a better understanding of protein function(1). As elementary constituents of cellular protein complexesand pathways, protein-protein interactions are key determinants of proteinfunction. Here we have built a large-scale protein-protein interaction mapof the human gastric pathogen Helicobacter pylori. We have used a high-throughput strategy of the yeast two-hybrid assay to screen 261 H. pylori proteins against a highly complex library of genome-encoded polypeptides(2). Over 1,200 interactions were identified between H. pylori proteins, connecting 46.6% of the proteome. The determination of a reliability score for everysingle protein-protein interaction and the identification of the actual interacting domains permitted the assignment of unannotated proteins to biological pathways.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:59:38