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Titolo:
Increased insulin-like growth factor 1 activity can rescue KLE endometrial-like cells from apoptosis
Autore:
Koutsilieris, M; Mastrogamvrakis, G; Lembessis, P; Sourla, A; Miligos, S; Michalas, S;
Indirizzi:
Univ Athens, Sch Med, Dept Expt Physiol, GR-11527 Athens, Greece Univ Athens Athens Greece GR-11527 Expt Physiol, GR-11527 Athens, Greece Platia Mavili, EndoOncoRes Labs, Athens, Greece Platia Mavili Athens Greece ia Mavili, EndoOncoRes Labs, Athens, Greece Univ Athens, Sch Med, Dept Obstet & Gynecol, GR-11527 Athens, Greece Univ Athens Athens Greece GR-11527 et & Gynecol, GR-11527 Athens, Greece
Titolo Testata:
MOLECULAR MEDICINE
fascicolo: 1, volume: 7, anno: 2001,
pagine: 20 - 26
SICI:
1076-1551(200101)7:1<20:IIGF1A>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-BINDING PROTEIN-3; BREAST-CANCER CELLS; PERITONEAL-FLUID; ENVIRONMENTAL TOXINS; PROSTATE-CANCER; WOMEN; EXPRESSION; IGF; TISSUE; INTERLEUKIN-8;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Koutsilieris, M Univ Athens, Sch Med, Dept Expt Physiol, GR-11527 Athens, Greece Univ Athens Athens Greece GR-11527 -11527 Athens, Greece
Citazione:
M. Koutsilieris et al., "Increased insulin-like growth factor 1 activity can rescue KLE endometrial-like cells from apoptosis", MOL MED, 7(1), 2001, pp. 20-26

Abstract

Background: The peritoneal fluid (PF) of women with endometriosis containsprotease(s) activity able to hydrolyze insulin-like growth factor-binding protein 3 (IGFBP-3), increasing the bioavailability of insulin-like growth factor-1 (IGF-1) locally. Therefore, we characterized the effects of IGF-1 on KLE endometrial-like cells in vitro. Materials and Methods: The mitogenic effect of IGF-1 was assessed by the analysis of the DNA content and cell count. Apoptosis was triggered experimentally by the 48 hr exposure of KLE cells to 100 nM of adriamycin in the presence and absence of IGF-1 (50 ng/ml). Adriamycin apoptosis of KLE cells was determined by the number of dead KLE cells using trypan blue exclusion and by the DNA fragmentation on simple agarose gel and flow cytometry of propidium iodide and HOECHST 33342-stained KLE cells using an EPICS 753 pulse cytometer. Results: ICE-I stimulated the growth of KLE cells in a dose-dependent manner (optimal dose of 50 ng/ml) and protected I(LE cells from adriamycin (100nM)-induced apoptosis. Conclusions: These data suggest that IGF-I is a survival factor for I(LE cells. Conceivably, increased IGF-1 activity in the PF can optimize both thesurvival and ectopic growth of endometrial cells in the peritoneal cavity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:15:28