Catalogo Articoli (Spogli Riviste)
OPAC HELP
Titolo: Estimating the probability of identity among genotypes in natural populations: cautions and guidelines
Autore: Waits, LP; Luikart, G; Taberlet, P;
 Indirizzi:
 Univ Idaho, Dept Fish & Wildlife Resources, Moscow, ID 83844 USA Univ Idaho Moscow ID USA 83844 & Wildlife Resources, Moscow, ID 83844 USA Univ Grenoble 1, CNRS, UMR 5553, Lab Biol Populat Altitude, F38041 Grenoble 9, France Univ Grenoble 1 Grenoble France 9 t Altitude, F38041 Grenoble 9, France
 Titolo Testata:
 MOLECULAR ECOLOGY
fascicolo: 1,
volume: 10,
anno: 2001,
pagine: 249  256
 SICI:
 09621083(200101)10:1<249:ETPOIA>2.0.ZU;2B
 Fonte:
 ISI
 Lingua:
 ENG
 Soggetto:
 WOMBAT LASIORHINUSKREFFTII; MICROSATELLITE ANALYSIS; GENETICVARIATION; BROWN BEARS; DNA; AFLP; HAIR; INDIVIDUALS; DIVERSITY; BLACK;
 Keywords:
 DNA fingerprinting; match probability; microsatellites; noninvasive genetic sampling; population estimation; probability of identity;
 Tipo documento:
 Article
 Natura:
 Periodico
 Settore Disciplinare:
 Agriculture,Biology & Environmental Sciences
 Life Sciences
 Citazioni:
 46
 Recensione:
 Indirizzi per estratti:
 Indirizzo: Waits, LP Univ Idaho, Dept Fish & Wildlife Resources, Moscow, ID 83844 USAUniv Idaho Moscow ID USA 83844 Resources, Moscow, ID 83844 USA



 Citazione:
 L.P. Waits et al., "Estimating the probability of identity among genotypes in natural populations: cautions and guidelines", MOL ECOL, 10(1), 2001, pp. 249256
Abstract
Individual identification using DNA fingerprinting methods is emerging as a critical tool in conservation genetics and molecular ecology. Statisticalmethods that estimate the probability of sampling identical genotypes using theoretical equations generally assume random associations between alleles within and among loci. These calculations are probably inaccurate for many animal and plant populations due to population substructure. We evaluatedthe accuracy of a probability of identity (P(ID)) estimation by comparingthe observed and expected P(ID)), using large nuclear DNA microsatellite data sets from three endangered species: the grey wolf (Canis lupus), the brown bear (Ursus arctos), and the Australian northern hairynosed wombat (Lasiorinyus krefftii). The theoretical estimates of P(ID), were consistently lower than the observed P(ID) and can differ by as much as three orders of magnitude. To help researchers and managers avoid potential problems associated with this bias, we introduce an equation for P(ID) between sibs. This equation provides an estimator that can be used as a conservative upperbound for the probability of observing identical multilocus genotypes between two individuals sampled from a population. We suggest computing the actual observed P(ID) when possible and give general guidelines for the number of codominant and dominant marker loci required to achieve a reasonably low P(ID) (e.g. 0.010.0001).
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 08:07:10