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Titolo:
Nerve growth factor down-regulates the expression of norepinephrine transporter in rat pheochromocytoma (PC12) cells
Autore:
Ikeda, T; Kitayama, S; Morita, K; Dohi, T;
Indirizzi:
Hiroshima Univ, Fac Dent, Dept Pharmacol, Minami Ku, Hiroshima 7348553, Japan Hiroshima Univ Hiroshima Japan 7348553 nami Ku, Hiroshima 7348553, Japan
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 1-2, volume: 86, anno: 2001,
pagine: 90 - 100
SICI:
0169-328X(20010131)86:1-2<90:NGFDTE>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
OCT-2 TRANSCRIPTION FACTOR; ADRENAL CHROMAFFIN CELLS; TYROSINE-HYDROXYLASE; DOPAMINE TRANSPORTER; GENE-EXPRESSION; MESSENGER-RNA; NORADRENALINE; SEQUENCES; PROTEIN; NEURONS;
Keywords:
nerve growth factor; norepinephrine transporter; PC12 cells; tyrosine hydroxylase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Kitayama, S Hiroshima Univ, Fac Dent, Dept Pharmacol, Minami Ku, Kasumi 1-2-3, Hiroshima 7348553, Japan Hiroshima Univ Kasumi 1-2-3 Hiroshima Japan 7348553 53, Japan
Citazione:
T. Ikeda et al., "Nerve growth factor down-regulates the expression of norepinephrine transporter in rat pheochromocytoma (PC12) cells", MOL BRAIN R, 86(1-2), 2001, pp. 90-100

Abstract

Functional expression of norepinephrine transporter (NET) and its regulation were examined in rat pheochromocytoma cell line, PC12. Nerve growth factor (NGF) decreased [H-3]-norepinephrine (NE) uptake in association with a decrease in NET mRNA levels. On the other hand, levels of tyrosine hydroxylase mRNA increased in PC12 cells treated with NGF for 4-24 h, while Oct-2 mRNA levels decreased at 4 h with NGF then recovered for 8-24 h in the presence of NGF. Both bFGF and EGF reduced [H-3]NE uptake, although they failed to affect NET mRNA levels. To examine the NET transcriptional regulation, weidentified the 5'-noncoding region of rat NET mRNA by the rapid amplification of cDNA end (RACE) method and cloned the 5/-flanking region of NET gene. The newly identified exon encodes the untranslated region of rat NET mRNAupstream of the known 5'-region including ATG stare codon. Constructs having green fluorescent protein (GFP) as reporter were made with the cloned NET gene, and promoter activity was examined in CHO and SK-N-SH cells transiently transfected and in PC12 cells stably transfected with NET-GFP constructs. The results indicate that the 2.1 kb NET flanking region displays promoter activity and is responsible for the NGF-induced down-regulation of NET expression. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 23:42:05