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Titolo:
Identification of four novel human G protein-coupled receptors expressed in the brain
Autore:
Lee, DK; George, SR; Cheng, R; Nguyen, T; Liu, Y; Brown, M; Lynch, KR; ODowd, BF;
Indirizzi:
Univ Toronto, Dept Pharmacol, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 macol, Toronto, ON M5S 1A8, Canada Univ Toronto, Dept Med, Toronto, ON M5S 1A8, Canada Univ Toronto Toronto ON Canada M5S 1A8 t Med, Toronto, ON M5S 1A8, Canada Ctr Addict & Mental Hlth, Toronto, ON M5S 2S1, Canada Ctr Addict & Mental Hlth Toronto ON Canada M5S 2S1 to, ON M5S 2S1, Canada Univ Virginia, Hlth Sci Ctr, Dept Pharmacol, Charlottesville, VA 22908 USAUniv Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 1-2, volume: 86, anno: 2001,
pagine: 13 - 22
SICI:
0169-328X(20010131)86:1-2<13:IOFNHG>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANIN-CONCENTRATING-HORMONE; ENDOGENOUS LIGAND; UROTENSIN-II; ORPHAN RECEPTOR; OPIOID RECEPTOR; NATURAL LIGAND; C5A RECEPTOR; CLONING; BINDING; PEPTIDE;
Keywords:
G protein-coupled receptor; orphan; intronless;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: O'Dowd, BF Univ Toronto, Dept Pharmacol, Med Sci Bldg, Toronto, ON M5S 1A8, Canada Univ Toronto Med Sci Bldg Toronto ON Canada M5S 1A8 1A8, Canada
Citazione:
D.K. Lee et al., "Identification of four novel human G protein-coupled receptors expressed in the brain", MOL BRAIN R, 86(1-2), 2001, pp. 13-22

Abstract

We report the discovery and tissue distributions of four novel human genes, GPR61, GPR62, GPR63 and GPR77, all of which encode G protein-coupled receptors (GPCRs), GPR61 was discovered in a search of the patent literature which retrieved a rabbit DNA sequence partially encoding a novel GPCR. This sequence was used to obtain a full-length human cDNA encoding GPR61, a receptor of 417 amino acid length. A search of the GenBank genomic sequence databases revealed three previously unrecognized intronless genes encoding the orphan GPCrs (oGPCRs) GPR62, GPR63 and GPR77, with respective amino acid lengths of 368, 419 and 337. Sequence analysis revealed that GPR61 and GPR62,and a published orphan receptor p47MNR, shared the highest level of identities to each other, ranging from 36 to 45% in the transmembrane (TM) domains. Together, these three oGPCRs appear to comprise a novel subfamily of GPCRs, most closely related to the serotonin 5-HT6 receptor. Sequence analysisof GPR63 and GPR77 revealed highest sequence identities in the TM regions with the oGPCR PSP24 (58%) and the anaphylatoxin C5a receptor (49%) respectively. Tissue distribution analyses detected the expression of all four novel genes in the human brain. GPR61 mRNA expression was detected in the caudate, putamen and thalamus of human brain, with a more widespread expressionpattern in rat brain, with mRNA signals in areas of the cortex, hippocampus, thalamus, hypothalamus and midbrain. GPR62 mRNA expression was detected in the basal forebrain, frontal cortex, caudate, putamen, thalamus and hippocampus. GPR63 mRNA expression was detected in the frontal cortex, with lower levels in the thalamus, caudate, hypothalamus and midbrain. Analysis of GPR77 mRNA expression revealed signals in the frontal cortex, hippocampus and hypothalamus with high transcript levels in the liver. (C) 2001 ElsevierScience B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 01:51:06