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Titolo:
Functional characterization of two splice variants of rat bad and their interaction with Bcl-w in sympathetic neurons
Autore:
Hamner, S; Arumae, U; Li-Ying, Y; Sun, YF; Saarma, M; Lindholm, D;
Indirizzi:
Univ Uppsala, BMC, Dept Neurosci & Neurobiol, S-75123 Uppsala, Sweden UnivUppsala Uppsala Sweden S-75123 & Neurobiol, S-75123 Uppsala, Sweden Univ Helsinki, Inst Biotechnol, Bioctr 1, Helsinki 00014, Finland Univ Helsinki Helsinki Finland 00014 , Bioctr 1, Helsinki 00014, Finland
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 1, volume: 17, anno: 2001,
pagine: 97 - 106
SICI:
1044-7431(200101)17:1<97:FCOTSV>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; X MESSENGER-RNA; CYTOCHROME-C; DIFFERENTIAL EXPRESSION; FAMILY PROTEINS; NERVOUS-SYSTEM; APOPTOSIS; SURVIVAL; MITOCHONDRIA; REGULATOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Lindholm, D Univ Uppsala, BMC, Dept Neurosci & Neurobiol, Box 587, S-75123Uppsala, Sweden Univ Uppsala Box 587 Uppsala Sweden S-75123 3 Uppsala, Sweden
Citazione:
S. Hamner et al., "Functional characterization of two splice variants of rat bad and their interaction with Bcl-w in sympathetic neurons", MOL CELL NE, 17(1), 2001, pp. 97-106

Abstract

Neuronal cell death is in many cases regulated by competitive interactionsbetween pro- and antiapoptotic proteins of the Bcl-2 family. In this studywe have identified two splice variants of the rat proapoptotic molecule Bad, which differ in their carboxy-terminal regions. Both splice variants of Bad interacted with the antiapoptotic molecule Bcl-w as shown by yeast two-hybrid assay and by coimmunoprecipitation experiments from transfected cells. mRNA expression for the two variants of bad were detected in all neonatal and adult rat tissues tested. Overexpression of either of the two isoforms of Bad in nerve growth factor (NGF)-maintained sympathetic neurons by microinjection induced the cell death of these neurons, which was neutralized by co-expression of Bcl-w. Overexpression of Bcl-w in sympathetic neurons also counteracted death induced by NGF deprivation, which was not reduced bycc-expression of either of the two Bad variants. The results suggest that Bcl-w, Bad-alpha, and Bad-beta may participate in the regulation of apoptosis in the sympathetic nervous system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 09:08:09