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Titolo:
The chum salmon insulin-like growth factor II promoter requires Sp1 for its activation by C/EBP beta
Autore:
Palamarchuk, AY; Kavsan, VM; Sussenbach, JS; Holthuizen, PE;
Indirizzi:
Univ Utrecht, Med Ctr, Physiol Chem Lab, NL-3584 CG Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 CG 3584 CG Utrecht, Netherlands Natl Acad Sci Ukraine, Inst Mol Biol & Genet, Dept Biosynthesis Nucl Acids, UA-252627 Kiev, Ukraine Natl Acad Sci Ukraine Kiev Ukraine UA-252627 ds, UA-252627 Kiev, Ukraine
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 172, anno: 2001,
pagine: 57 - 67
SICI:
0303-7207(20010214)172:1-2<57:TCSIGF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING-PROTEIN-ALPHA; FACTOR MESSENGER-RNA; TRANSCRIPTION FACTOR; PARTIAL-HEPATECTOMY; GENE; EXPRESSION; LIVER; TELEOST; FAMILY; TISSUE;
Keywords:
transcription regulation; IGF-II; gene expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Holthuizen, PE Univ Utrecht, Med Ctr, Physiol Chem Lab, Universiteitsweg 100, NL-3584 CG Utrecht, Netherlands Univ Utrecht Universiteitsweg 100 Utrecht Netherlands NL-3584 CG
Citazione:
A.Y. Palamarchuk et al., "The chum salmon insulin-like growth factor II promoter requires Sp1 for its activation by C/EBP beta", MOL C ENDOC, 172(1-2), 2001, pp. 57-67

Abstract

The chum salmon insulin-like growth factor II (IGF-II) gene is highly expressed in liver tissue. In this study we demonstrate that two transcription factors, Sp1 and C/EBP beta, are involved in the enhanced expression of thesalmon IGF-II gene. The presence of the fish homolog for C/EBP beta in salmon liver RNA was confirmed by Northern blotting. The sIGF-II promoter was activated up to 20-fold by co-transfection with C/EBP beta. The functional importance of four out of the five putative C/EBP beta binding sites was demonstrated with mutational analysis in transient transfection assays. The transcription factor Sp1 binds to two sites within the salmon IGF-II promoter. Interestingly, mutation of the Sp1 binding sites decreases not only the basal IGF-II promoter activity but also the C/EBP beta -induced transactivation. These results demonstrate that liver-enriched C/EBP beta and ubiquitously expressed Sp1 each activate the sIGF-II promoter and that Sp1 is required for full transactivation of the sIGF-II gene by C/EBP beta. This suggests that C/EBP beta and Spl act in synergy. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

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Documento generato il 02/04/20 alle ore 22:21:02