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Titolo:
Immunocytochemical analysis of connexin expression in the healthy and diseased cardiovascular system
Autore:
Severs, NJ; Rothery, S; Dupont, E; Coppen, SR; Yeh, HI; Ko, YS; Matsushita, T; Kaba, R; Halliday, D;
Indirizzi:
Royal Brompton Hosp, Imperial Coll, Sch Med, Natl Heart & Lung Inst, London SW3 6NP, England Royal Brompton Hosp London England SW3 6NP Inst, London SW3 6NP, England
Titolo Testata:
MICROSCOPY RESEARCH AND TECHNIQUE
fascicolo: 3, volume: 52, anno: 2001,
pagine: 301 - 322
SICI:
1059-910X(20010201)52:3<301:IAOCEI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; GAP JUNCTION PROTEIN; SCANNING CONFOCAL MICROSCOPY; HUMAN HELA-CELLS; VENTRICULAR MYOCARDIUM; ENDOTHELIAL-CELLS; CONDUCTION SYSTEM; BORDER ZONE; INTERCELLULAR COMMUNICATION; CONNEXIN40-DEFICIENT MICE;
Keywords:
gap junctions; heart; myocyte; smooth muscle cell; endothelium; freeze-fracture cytochemistry; confocal microscopy;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
104
Recensione:
Indirizzi per estratti:
Indirizzo: Severs, NJ Royal Brompton Hosp, Imperial Coll, Sch Med, Natl Heart & Lung Inst, Sydney St, London SW3 6NP, England Royal Brompton Hosp Sydney St London England SW3 6NP , England
Citazione:
N.J. Severs et al., "Immunocytochemical analysis of connexin expression in the healthy and diseased cardiovascular system", MICROSC RES, 52(3), 2001, pp. 301-322

Abstract

Gap junctions play essential roles in the normal function of the heart andarteries, mediating the spread of the electrical impulse that stimulates synchronized contraction of the cardiac chambers, and contributing to co-ordination of activities between cells of the arterial wall. In common with other multicellular systems, cardiovascular tissues express multiple connexinisotypes that confer distinctive channel properties. This review highlights how state-of-the-art immunocytochemical and cellular imaging techniques, as part of a multidisciplinary approach in gap junction research, have advanced our understanding of connexin diversity in cardiovascular cell function in health and disease. In the heart, spatially defined patterns of expression of three connexin isotypes-connexin43, connexin40, and connexin45-underlie the precisely orchestrated patterns of current flow governing the normal cardiac rhythm. Derangement of gap junction organization and/or reduced expression of connexin43 are associated with arrhythmic tendency in the diseased human ventricle, and high levels of connexin40 in the atrium are associated with increased risk of developing atrial fibrillation after coronaryby-pass surgery. In the major arteries, endothelial gap junctions may simultaneously express three connexin isotypes, connexin40, connexin37, and connexin43; underlying medial smooth muscle, by contrast, predominantly expresses connexin43, with connexin45 additionally expressed at restricted sites. In normal arterial smooth muscle, the abundance of connexin43 gap junctions varies according to vascular site, and shows an inverse relationship withdesmin expression and positive correlation with the quantity of extracellular matrix. Increased connexin43 expression between smooth muscle cells is closely linked to phenotypic transformation in early human coronary atherosclerosis and in the response of the arterial wall to injury. Current evidence thus suggests that gap junctions in both their guises, as pathways for cell-to-cell signaling in the vessel wall and as pathways for impulse conduction in the heart, contribute to the initial pathogenesis and eventual clinical manifestation of human cardiovascular disease. Microsc. Res. Tech. 52:301-322, 2001. (C) 2001 Wiley-Liss. Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 19:01:47