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Titolo:
Detection of secondary genetic aberrations in follicle center cell derivedlymphomas: assessment of the reliability of comparative genomic hybridization and standard chromosome analysis
Autore:
Viardot, A; Martin-Subero, JI; Siebert, R; Harder, S; Gesk, S; Bentz, M; Schlegelberger, B;
Indirizzi:
Univ Kiel, Inst Human Genet, D-24105 Kiel, Germany Univ Kiel Kiel Germany D-24105 , Inst Human Genet, D-24105 Kiel, Germany Univ Ulm, Med Klin 3, Ulm, Germany Univ Ulm Ulm GermanyUniv Ulm, Med Klin 3, Ulm, Germany Univ Navarra, Dept Genet, E-31080 Pamplona, Spain Univ Navarra Pamplona Spain E-31080 Dept Genet, E-31080 Pamplona, Spain
Titolo Testata:
LEUKEMIA
fascicolo: 1, volume: 15, anno: 2001,
pagine: 177 - 183
SICI:
0887-6924(200101)15:1<177:DOSGAI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LYMPHOBLASTIC-LEUKEMIA; IN-SITU HYBRIDIZATION; CYTOGENETIC ANALYSIS; ABNORMALITIES; LOSSES; GAINS; FISH;
Keywords:
follicle center lymphoma; comparative genomic hybridization; standard chromosome analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Schlegelberger, B Univ Kiel, Inst Human Genet, Schwanenweg 24, D-24105 Kiel, Germany Univ Kiel Schwanenweg 24 Kiel Germany D-24105 , Germany
Citazione:
A. Viardot et al., "Detection of secondary genetic aberrations in follicle center cell derivedlymphomas: assessment of the reliability of comparative genomic hybridization and standard chromosome analysis", LEUKEMIA, 15(1), 2001, pp. 177-183

Abstract

Secondary chromosomal aberrations in follicle center cell derived lymphomas (FCDL) usually involve gains and losses of genetic material and may be animportant prognostic value. In the present study, we aimed to determine the power of comparative genomic hybridization (CGH) as compared to standard chromosome analysis (CA) to detect such secondary aberrations. The same lymph node cell suspensions prepared from 30 patients with FCDL were analyzed in parallel by CGH and CA based on R banding. In all, 73 discrepancies werefound. Sixty-two imbalances were detected only by CA and 11 only by CGH, In cases with completely resolved karyotypes (n = 17), the median number of discrepancies between CGH and CA was one. However, when the karyotype was partially resolved (n = 12), the median was four (P < 0.01), Discrepant results were further studied by fluorescence in situ hybridization using locus-specific probes. These data confirm, that not only for the detection of balanced aberrations, but also for the detection of unbalanced aberrations in FCDL, standard chromosome analysis is still the 'gold standard'. In contrast, CGH is useful to detect chromosomal imbalances when no metaphases are found or no fresh material is available.

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Documento generato il 28/09/20 alle ore 11:59:45