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Titolo:
The inositol 5-phosphatase SHIP is expressed as 145 and 135 kDa proteins in blood and bone marrow cells in vivo, whereas carboxyl-truncated forms of SHIP are generated by proteolytic cleavage in vitro
Autore:
Horn, S; Meyer, J; Heukeshoven, J; Fehse, B; Schulze, C; Li, S; Frey, J; Poll, S; Stocking, C; Jucker, M;
Indirizzi:
Univ Hamburg, Hosp Eppendorf, Abt Zellulare Signaltransdukt, Inst Med Biochem & Mol Biol, D-20246 Hamburg, Germany Univ Hamburg Hamburg Germany D-20246 Mol Biol, D-20246 Hamburg, Germany Univ Hamburg, Heinrich Pette Inst Expt Virol & Immunol, D-20246 Hamburg, Germany Univ Hamburg Hamburg Germany D-20246 & Immunol, D-20246 Hamburg, Germany Univ Hamburg, Hosp Eppendorf, Knochenmark Transplantat Zentrum, D-20246 Hamburg, Germany Univ Hamburg Hamburg Germany D-20246 t Zentrum, D-20246 Hamburg, Germany Univ Hamburg, Zentrum Mol Neurobiol, Hamburg, Germany Univ Hamburg Hamburg Germany g, Zentrum Mol Neurobiol, Hamburg, Germany Univ Bielefeld, D-4800 Bielefeld, Germany Univ Bielefeld Bielefeld Germany D-4800 efeld, D-4800 Bielefeld, Germany
Titolo Testata:
LEUKEMIA
fascicolo: 1, volume: 15, anno: 2001,
pagine: 112 - 120
SICI:
0887-6924(200101)15:1<112:TI5SIE>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYPHOSPHATE 5-PHOSPHATASE; MULTIPLE CYTOKINES; HODGKINS-DISEASE; PHOSPHATASE SHIP; SELF-RENEWAL; RECEPTOR; SHC; DIFFERENTIATION; INTERLEUKIN-3; LINES;
Keywords:
SHIP; proteolysis; acute myeloid leukemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Jucker, M Univ Hamburg, Hosp Eppendorf, Abt Zellulare Signaltransdukt, Inst Med Biochem & Mol Biol, Martinistr 52, D-20246 Hamburg, Germany Univ Hamburg Martinistr 52 Hamburg Germany D-20246 urg, Germany
Citazione:
S. Horn et al., "The inositol 5-phosphatase SHIP is expressed as 145 and 135 kDa proteins in blood and bone marrow cells in vivo, whereas carboxyl-truncated forms of SHIP are generated by proteolytic cleavage in vitro", LEUKEMIA, 15(1), 2001, pp. 112-120

Abstract

The inositol polyphosphate 5-phosphatase SHIP plays an important role in negative signalling in B cells and mast cells and in the down-regulation of cytokine receptor-mediated signals in myeloid cells. SHIP is expressed as a145 kDa full-length protein and an isoform of 135 kDa due to alternative splicing. Additional smaller forms of SHIP which are truncated at the carboxy terminus have been described in bone marrow and peripheral blood mononuclear cells (PBMC). Our data demonstrate that human bone marrow cells and PBMC from healthy donors and patients with acute myeloid leukemia express the 145 kDa form of SHIP and low amounts of a 135 kDa form of SHIP in vivo whereas C-terminal-truncated SHIP proteins are generated by a PMSF-sensitive protease during the preparation of cell lysates in vitro. We have further characterized this protease and identified a proteolytic cleavage site in the human SHIP protein C-terminal to tryptophan residue 941. These data supporta physiological role for the 145 and 135 kDa forms of SHIP in bone marrow and peripheral blood cells from normal donors and patients with acute myeloid leukemia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 05:05:35