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Titolo:
Cellular splicing factor RAF-2p48/NPI-5/BAT1/UAP56 interacts with the influenza virus nucleoprotein and enhances viral RNA synthesis
Autore:
Momose, F; Basler, CF; ONeill, RE; Iwamatsu, A; Palese, P; Nagata, K;
Indirizzi:
Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat,Lab Mol Med Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol Yokohama Kanagawa Japan 2268501 anagawa 2268501, Japan Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Kirin Brewery Co Ltd Yokohama Kanagawa Japan 2360004 agawa 2360004, Japan Mt Sinai Sch Med, Dept Microbiol, New York, NY 10029 USA Mt Sinai Sch MedNew York NY USA 10029 Microbiol, New York, NY 10029 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 4, volume: 75, anno: 2001,
pagine: 1899 - 1908
SICI:
0022-538X(200102)75:4<1899:CSFRIW>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR HISTOCOMPATIBILITY COMPLEX; A VIRUS; MESSENGER-RNA; VIRION RNA; NS1 PROTEIN; SACCHAROMYCES-CEREVISIAE; REPLICATION INVITRO; POLYMERASE ACTIVITY; KARYOPHERIN-ALPHA; FORK MODEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Nagata, K Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat,Lab Mol Med Engn, Midori Ku, 4259 Nagatsuta, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol 4259 Nagatsuta Yokohama Kanagawa Japan 2268501
Citazione:
F. Momose et al., "Cellular splicing factor RAF-2p48/NPI-5/BAT1/UAP56 interacts with the influenza virus nucleoprotein and enhances viral RNA synthesis", J VIROLOGY, 75(4), 2001, pp. 1899-1908

Abstract

Previous biochemical data identified a host cell fraction, designated RAF-2, which stimulated influenza virus RNA synthesis. A 48-kDa polypeptide (RAF-2p48), a cellular splicing factor belonging to the DEAD-box family of RNA-dependent ATPases previously designated BAT1 (also UAP56), has now been identified as essential for RAF-2 stimulatory activity. Additionally, RAF-2p48 was independently identified as an influenza virus nucleoprotein (NP)-interacting protein, NPI-5, in a yeast two-hybrid screen of a mammalian cDNA library. In vitro, RAP-2p48 interacted with free NP but not with NP bound toRNA, and the RAF-2p48-NP complex was dissociated following addition of free RNA. Furthermore, RAF-2p48 facilitated formation of the NP-RNA complexes that likely serve as templates for the viral RNA polymerase. RAF-2p48 was shown, in both in vitro binding assays and the yeast two-hybrid system, to bind to the amino-terminal region of NP, a domain essential for RNA binding. Together, these observations suggest that RAF-2p48 facilitates NP-RNA interaction, thus leading to enhanced influenza virus RNA synthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 09:20:14