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Titolo:
Human immunodeficiency virus (HIV)-specific immune responses are generatedwith the simultaneous vaccination of a gp120-depleted, whole-killed HIV-1 immunogen with cytosine-phosphorothioate-guanine dinucleotide immunostimulatory sequences of DNA
Autore:
Moss, RB; Diveley, J; Jensen, FC; Gouveia, E; Carlo, DJ;
Indirizzi:
Immune Response Corp, Carlsbad, CA 92008 USA Immune Response Corp Carlsbad CA USA 92008 e Corp, Carlsbad, CA 92008 USA
Titolo Testata:
JOURNAL OF HUMAN VIROLOGY
fascicolo: 1, volume: 4, anno: 2001,
pagine: 39 - 43
SICI:
1090-9508(200101/02)4:1<39:HIV(IR>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-CHEMOKINES; ADJUVANTS; INFECTION; AIDS;
Keywords:
HIV-specific immune responses; simultaneous vaccination; preventive vaccine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Moss, RB Immune Response Corp, 5935 Darwin Court, Carlsbad, CA 92008 USA Immune Response Corp 5935 Darwin Court Carlsbad CA USA 92008 USA
Citazione:
R.B. Moss et al., "Human immunodeficiency virus (HIV)-specific immune responses are generatedwith the simultaneous vaccination of a gp120-depleted, whole-killed HIV-1 immunogen with cytosine-phosphorothioate-guanine dinucleotide immunostimulatory sequences of DNA", J HUMAN VIR, 4(1), 2001, pp. 39-43

Abstract

Objectives: We compared the effect of priming with a synthetic oligodeoxynudeotide (ODN) immunostimulatory DNA sequence followed by vaccination with human immunodeficiency virus type 1 (HIV-1) in incomplete Freund's adjuvant(IFA) or HIV-1 antigen alone to the simultaneous administration of immunostimulatory sequences (ISS) with HIV-1 in IFA. Methods: We examined immune function involving interferon-gamma (IFN-gamma) production, RANTES (regulated upon activation, normal T cell expressed and secreted) production, and lymphocyte proliferation, all of which appear to be augmented in HIV-1-exposed, but uninfected, individuals. Results: We demonstrate that similar levels of antigen-specific IFN-gamma were produced from lymph node cells of the animals immunized with HIV-1 antigen in IFA containing the CpG ODN 1826 (ISS; mean +/- SE = 450.8 +/- 224.3pg/mL) and the group of animals primed with the ODN before injection with the HIV-1 in IFA (mean +/- SE = 377.7 +/- 294.8 pg/mL) or HIV-1 antigen alone (IFN-gamma = 0 pg/mL). However, the group that received the HIV-1 in IFAplus ISS mounted a stronger lymphocyte proliferation (mean net +/- SE = 29,180 +/- 1,932 cpm) compared to the group primed with the ODN before injection with HIV-1 in IFA (mean net +/- SE = 8,575 +/- 2,978 cpm). Furthermore,the group that received the HIV-1 in IFA plus ISS also mounted stronger beta -chemokine production measured as RANTES (mean +/- SE = 1.217 +/- 267.4 pg/mL) compared to the group that received the ODN before injection with HIV-1 in IFA (mean +/- SE = 129.1 +/- 48.5 pg/mL). antibody responses from the group that received the HIV-1 in IFA plus ISS also showed a higher p24-specific response that was predominantly of the immunoglobulin G IgG2b isotype. Conclusion: These results suggest that the simultaneous administration of the ISS in the HIV-1 in IFA emulsion may be a condidate for testing in non-human primates and in human studies as a therapeutic and preventative vaccine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:20:41