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Titolo:
Expression of hepatocyte growth factor in human hepatocellular carcinoma
Autore:
Guirouilh, J; Le Bail, B; Boussarie, L; Balabaud, C; Bioulac-Sage, P; Desmouliere, A; Schuppan, D; Rosenbaum, J;
Indirizzi:
Univ Bordeaux 2, Grp Rech Etud Foie, INSERM E9917, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 E9917, F-33076 Bordeaux, France Univ Erlangen Nurnberg, Dept Gastroenterol, D-91054 Erlangen, Germany UnivErlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany
Titolo Testata:
JOURNAL OF HEPATOLOGY
fascicolo: 1, volume: 34, anno: 2001,
pagine: 78 - 83
SICI:
0168-8278(200101)34:1<78:EOHGFI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR SCATTER FACTOR; RECEPTOR C-MET; TRANSGENIC MICE; MESSENGER-RNA; IN-VITRO; LIVER; CELLS; OVEREXPRESSION; MYOFIBROBLASTS; INVASIVENESS;
Keywords:
myofibroblasts; stellate cells; cancer; growth factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Rosenbaum, J Univ Bordeaux 2, Grp Rech Etud Foie, INSERM E9917, 146 Rue Leo Saignat, F-33076 Bordeaux, France Univ Bordeaux 2 146 Rue Leo Saignat Bordeaux France F-33076
Citazione:
J. Guirouilh et al., "Expression of hepatocyte growth factor in human hepatocellular carcinoma", J HEPATOL, 34(1), 2001, pp. 78-83

Abstract

Background/Aims: We have shown that hepatocyte growth factor, secreted by human liver myofibroblasts, promoted in vitro invasion of human hepatocellular carcinoma cell lines. The aim of this work was to measure hepatocyte growth factor expression in 29 human hepatocellular carcinomas and the corresponding peri-tumoral livers. Methods: We used reverse transcription-polymerase chain reaction, in situ hybridization, ELISA and Western blot. Results: Sixty-two of tested hepatocellular carcinomas were positive by reverse transcription-polymerase chain reaction. With in situ hybridization, a signal was found in every sample. In many cases, the signal was localizedin cells labeled with an anti-smooth muscle alpha -actin antibody, while hepatocytes were mostly non-labeled. ELISA, performed in 15 pairs of hepatocellular carcinomas and surrounding livers, detected hepatocyte growth factor in every sample with wide variations. Hepatocellular carcinomas that had developed in non-cirrhotic livers contained essentially the same amount of hepatocyte growth factor as the matching non-tumoral liver, In cirrhotic livers, the hepatocyte growth factor content of the tumors was significantly lower than that of the surrounding cirrhotic livers. Conclusions: These data indicate that hepatocyte growth factor is expressed at significant levels in every hepatocellular carcinoma tested and that its expression takes place in the stromal myofibroblasts. (C) 2001 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:07:59