Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The salivary apyrase of the blood-sucking sand fly Phlebotomus papatasi belongs to the novel Cimex family of apyrases
Autore:
Valenzuela, JG; Belkaid, Y; Rowton, E; Ribeiro, JMC;
Indirizzi:
NIAID, Sect Med Entomol, Parasit Dis Lab, Bethesda, MD 20892 USA NIAID Bethesda MD USA 20892 omol, Parasit Dis Lab, Bethesda, MD 20892 USA Walter Reed Army Med Ctr, Walter Reed Army Inst Res, Dept Entomol, Washington, DC 20307 USA Walter Reed Army Med Ctr Washington DC USA 20307 Washington, DC 20307 USA
Titolo Testata:
JOURNAL OF EXPERIMENTAL BIOLOGY
fascicolo: 2, volume: 204, anno: 2001,
pagine: 229 - 237
SICI:
0022-0949(200101)204:2<229:TSAOTB>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOSQUITO ANOPHELES-ALBIMANUS; RHODNIUS-PROLIXUS; AEDES-AEGYPTI; LUTZOMYIA-LONGIPALPIS; PURIFICATION; CLONING; NITROPHORIN; LECTULARIUS; ATPASES; BINDING;
Keywords:
apyrase; salivary gland; haematophagy; bed bug; Cimex lectularius;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Ribeiro, JMC NIAID, Sect Med Entomol, Parasit Dis Lab, Bldg 4,Room 126,4 Ctr Dr,MSC 0425, Bethesda, MD 20892 USA NIAID Bldg 4,Room 126,4 Ctr Dr,MSC 0425 Bethesda MD USA 20892
Citazione:
J.G. Valenzuela et al., "The salivary apyrase of the blood-sucking sand fly Phlebotomus papatasi belongs to the novel Cimex family of apyrases", J EXP BIOL, 204(2), 2001, pp. 229-237

Abstract

Apyrases are enzymes that hydrolyze nucleotide di- and triphosphates to orthophosphate and mononucleotides, At least two families of enzymes, belonging to the 5'-nucleotidase and to the actin/heat shock 70/sugar kinase superfamily, have evolved independently to serve the apyrase reaction. Both families require either Ca2+ or Mg2+ for their action. A novel apyrase enzyme sequence, with no homology to any other known protein sequence, was found recently in the salivary glands of the hematophagous bed bug Cimex lectularius. This enzyme functions exclusively with Ca2+. Here, we report the findingof a cDNA similar to that of the C. lectularius salivary apyrase isolated from a salivary gland cDNA library of Phlebotomus papatasi, Transfection ofinsect cells with the P. papatasi salivary gland apyrase cDNA resulted in the secretion of a Ca2+-dependent apyrase whose activity was indistinguishable from that in salivary homogenates of P. papatasi. Homologous sequences were found in humans, in another sand fly (Lutzomyia longipalpis), in the fruit fly Drosophila melanogaster, in the nematode Caenorhabditis elegans and in the protozoan Cryptosporidium parvum, indicating that this family of enzymes is widespread among animal species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 09:59:53