Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Strategy for the maximization of clinically relevant information from hepatitis C virus, RT-PCR quantification
Autore:
Levis, J; Kenny-Walsh, E; OSullivan, K; Horgan, M; Whelton, M; Shanahan, F; Fanning, L;
Indirizzi:
Univ Coll Cork, Hepatitis C Unit, Dept Med, Cork Univ Hosp, Cork, Ireland Univ Coll Cork Cork Ireland it, Dept Med, Cork Univ Hosp, Cork, Ireland Univ Coll Cork, Dept Stat, Cork, Ireland Univ Coll Cork Cork IrelandUniv Coll Cork, Dept Stat, Cork, Ireland
Titolo Testata:
JOURNAL OF CLINICAL VIROLOGY
fascicolo: 3, volume: 20, anno: 2001,
pagine: 163 - 171
SICI:
1386-6532(200102)20:3<163:SFTMOC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA SIGNAL AMPLIFICATION; HCV-RNA; INTERFERON TREATMENT; PROBE ASSAY; RIBAVIRIN; COMBINATION; INDIVIDUALS; INFECTION; FEATURES; DISEASE;
Keywords:
hepatitis C virus; viral load; quantification; linear range; PCR; cost benefit;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Fanning, L Univ Coll Cork, Hepatitis C Unit, Dept Med, Cork Univ Hosp, Clin Sci Bldg,Cork, Ireland Univ Coll Cork Clin Sci Bldg Cork Ireland Bldg,Cork, Ireland
Citazione:
J. Levis et al., "Strategy for the maximization of clinically relevant information from hepatitis C virus, RT-PCR quantification", J CLIN VIRO, 20(3), 2001, pp. 163-171

Abstract

Background: The increasing clinical application of viral load assays for monitoring viral infections has been an incentive for the development of standardized tests for the hepatitis C virus. Objective : To develop a simple model for the prediction of baseline viral load in individuals infected with the hepatitis C virus. Methodology: Viral load quantification of each patient's first sample was assessed by RT-PCR-ELISA using the Roche MONITOR assay in triplicate. Genotype of the infecting virus was identified by reverse line probe hybridization. using amplicons resulting from the qualitative HCV Roche AMPLICOR assay. Results: Retrospective evaluation of first quantitative values suggested that 82.4% (n = 168/204) of individuals had a viralload between 4.3 and 6.7 log(10) viral copies per mi. A few patients (3.4%; n = 7/204) have a serum viremia less than the lower limit of the linear range of the RT-PCR assay. Subsequent, prospective evaluation of hepatitis Cviral load of all new patients: using a model based on the dynamic range of viral load in the retrospective group correctly predicted the dynamic range in 75.9% (n = 33/54). Conclusion: The dynamic range of hepatitis C viremia extends beyond the linear range of the Roche MONITOR assay. Accurate determination of serum viremia is substantially improved by dilution of specimens prior to quantification. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:15:57