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Titolo:
Convergence of alpha(v)beta(3) integrin- and macrophage colony stimulatingfactor-mediated signals on phospholipase C gamma in prefusion osteoclasts
Autore:
Nakamura, I; Lipfert, L; Rodan, GA; Duong, LT;
Indirizzi:
Merck Res Labs, Dept Bone Biol & Osteoporosis Res, W Point, PA 19486 USA Merck Res Labs W Point PA USA 19486 teoporosis Res, W Point, PA 19486 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 2, volume: 152, anno: 2001,
pagine: 361 - 373
SICI:
0021-9525(20010122)152:2<361:COAIAM>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SRC-FAMILY KINASES; FOCAL ADHESION KINASE; GROWTH-FACTOR; TYROSINE PHOSPHORYLATION; SEALING ZONE; CELL-PROLIFERATION; PI-3 KINASE; FACTOR-I; ACTIVATION; RECEPTOR;
Keywords:
alpha(v)beta(3) integrins; osteoclasts; M-CSF; Src kinases; phospholipase C gamma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Duong, LT Merck Res Labs, Dept Bone Biol & Osteoporosis Res, W Point, PA 19486 USA Merck Res Labs W Point PA USA 19486 Res, W Point, PA 19486 USA
Citazione:
I. Nakamura et al., "Convergence of alpha(v)beta(3) integrin- and macrophage colony stimulatingfactor-mediated signals on phospholipase C gamma in prefusion osteoclasts", J CELL BIOL, 152(2), 2001, pp. 361-373

Abstract

The macrophage colony stimulating factor (M-CSF) and (alpha (v)beta (3) integrins play critical roles in osteoclast function. This study examines M-CSF- and adhesion-induced signaling in prefusion osteoclasts (pOCs) derived from Src-deficient and wild-type mice. Src-deficient cells attach to but donot spread on vitronectin (Vn)-coated surfaces and, contrary to wild-type cells, their adhesion does not lead to tyrosine phosphorylation of molecules activated by adhesion, including PYK2, p130(Cas), paxillin, and PLC-gamma. However, in response to M-CSF Src(-/-) pOCs spread and migrate on Vn in an alpha (v)beta (3)-dependent manner. Involvement of PLC-gamma activation is suggested by using a PLC inhibitor, U73122, which blocks both adhesion- and M-CSF-mediated cell spreading. Furthermore, in Src-/- pOCs M-CSF together with filamentous actin, causes recruitment of beta (3) integrin and PLC-gamma to adhesion contacts and induces stable association of beta (3) integrin with PLC-gamma, phosphatidylinositol 3-kinase, and PYK2. Moreover, direct interaction of PYK2 and PLC-gamma can be induced by either adhesion or M-CSF suggesting that this interaction may enable the formation of integrin-associated complexes. Furthermore, this study suggests that in pOCs PLC-gamma is a common downstream mediator for adhesion and growth factor signals. M-CSF-initiated signaling modulates the alpha (v)beta (3) integrin-mediated cytoskeletal reorganization in prefusion osteoclasts in the absence of c-Src, possibly via PLC-gamma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:35:04