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Titolo:
Genomic approaches to typing, taxonomy and evolution of bacterial isolates
Autore:
Gurtler, V; Mayall, BC;
Indirizzi:
Austin & Repatriat Med Ctr, Dept Microbiol, Heidelberg, Vic 3084, Australia Austin & Repatriat Med Ctr Heidelberg Vic Australia 3084 3084, Australia
Titolo Testata:
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
, volume: 51, anno: 2001,
parte:, 1
pagine: 3 - 16
SICI:
1466-5026(200101)51:<3:GATTTA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SITE-SPECIFIC INTEGRATION; 16S-23S RDNA SPACER; RESISTANT STREPTOCOCCUS-PNEUMONIAE; MULTILOCUS ENZYME ELECTROPHORESIS; ROLLING-CIRCLE AMPLIFICATION; LENGTH POLYMORPHISM ANALYSIS; FIELD GEL-ELECTROPHORESIS; ESCHERICHIA-COLI; STAPHYLOCOCCUS-AUREUS; RIBOSOMAL-RNA;
Keywords:
genome organization and analysis; genome polymorphism rate variations; genome evolution; specific gene variation; mobile genetic elements;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
140
Recensione:
Indirizzi per estratti:
Indirizzo: Gurtler, V Austin & Repatriat Med Ctr, Dept Microbiol, Austin Campus,Studley Rd, Heidelberg, Vic 3084, Australia Austin & Repatriat Med Ctr Austin Campus,Studley Rd Heidelberg Vic Australia 3084
Citazione:
V. Gurtler e B.C. Mayall, "Genomic approaches to typing, taxonomy and evolution of bacterial isolates", INT J SY EV, 51, 2001, pp. 3-16

Abstract

The current literature on bacterial taxonomy, typing and evolution will becritically examined from the perspective of whole-genome structure, function and organization. The following three categories of DNA band pattern studies will be reviewed: (i) random whole-genome analysis; (ii) specific genevariation and (iii) mobile genetic elements. (i) The use of RAPD, PFGE andAFLP to analyse the whole genome will provide a skeleton of polymorphic sites with exact genomic positions as whole-genome sequence data become available. (ii) Different genes provide different levels of evolutionary information for determining isolate relatedness depending on whether they are highly variable (prone to recombination events and horizontal transfer), housekeeping genes with only a small number of single nucleotide differences between isolates or part. of the rm multigene family that is prone to intragenomic recombination and concerted evolution. Comparative analyses of these different gene classes can provide enhanced information about isolate relatedness. (iii) Mobile genetic elements such as insertion sequences, transposons, plasmids and bacteriophages integrate into the bacterial genome at specific (e.g. tRNA genes) or non-specific sites to alter band patterns producedby PFGE, RAPD or AFLP. From the literature it is not clear what level of genetic element duplication constitutes non-relatedness of isolates. A modelis presented that incorporates all of the above genomic characteristics for the determination of isolate relatedness in taxonomic, typing and evolutionary studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 14:46:47